新型细菌菌株硝酸盐芽孢杆菌(B2)对酒厂污水的脱色作用

IF 0.7 Q4 MICROBIOLOGY Journal of Pure and Applied Microbiology Pub Date : 2023-11-20 DOI:10.22207/jpam.17.4.26
Ajad Patel, Ranjan Singh, R. Gaur
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引用次数: 0

摘要

甘蔗糖蜜在转化为乙醇的过程中,会产生大量生化需氧量(BOD)和化学需氧量(COD)较高的废水。这些废水中含有黑色素。黑色素是一种可被某些细菌分解的化学物质,可产生过氧化物酶、酚氧化酶、酵母酶、单氧酶和二氧酶。这些细菌的主要功能是分解复杂的碳氢化合物,包括芳香族化合物,如着色颜料。本研究旨在从自然资源中找出具有耐热性并有可能用于工业规模酒厂污水处理的黑色素脱色微生物。研究人员在固体培养基(包括糖蜜色素)上测试了 55 个不同的分离菌株。三种耐热细菌分离物被鉴定为类黑色素脱色剂:它们分别是硝酸芽孢杆菌(B2)、副黏液芽孢杆菌(B1)和布鲁氏菌(B3)。这些分离物在不同的物理化学和营养条件下进行了脱色优化。在 40°C、pH 值为 6.0 的条件下,使用 0.5% 葡萄糖(w/v)、0.5% 蛋白胨(w/v)、0.05% MgSO4 和 0.01% KH2PO4 在静态条件下培养 40 小时后,硝酸杆菌(B2)的脱色率(86%)在三个物种中最高。在浸没式发酵中,硝化细菌 B2 菌株可以承受更高的温度,并且只需要少量的碳源(0.5%,[w/v])和氮源(0.5%,[w/v])。因此,在工业规模上使用黑色素对蒸馏废水进行脱色是可行的。
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Decolorization of Distillery Effluent by the Novel Bacterial Strain Bacillus nitratireducens (B2)
As sugarcane molasses is converted into ethanol, a sizable volume of effluent with high biochemical oxygen demand (BOD) and chemical oxygen demand(COD) is generated. This effluent contains melanoidin. Melanodin is a chemical broken down by certain bacteria and can produce peroxidases, phenoxidases, laccases, and mono- and dioxygenases. The primary function of these bacteria is to break down complex hydrocarbons, including aromatics such as coloring pigments. This study aimed to identify melanoidin-decolorizing microorganisms in natural resources that are thermally resistant and have the potential to be used in industrial-scale distillery treatment for effluent applications. A total of 55 distinct isolates were tested on a solid medium, including molasses pigments. Three thermotolerant bacterial isolates were identified as melanoidin-decolorizing agents: Bacillus nitratireducens (B2), Bacillus paramycoides (B1), and Brucellatritici (B3). These isolates under went additional optimization for decolorization under various physicochemical and nutritional conditions. At 40°C, B. nitratireducens (B2) exhibited the highest degree of decolorization (86%) among the three species while using 0.5% glucose(w/v), 0.5% peptone(w/v), 0.05% MgSO4, and 0.01% KH2PO4 at a pH of 6.0 over 40 h of incubation under static conditions. In submerged fermentation, the B2 strain of B.nitratireducens can withstand higher temperatures and requires only a small amount of carbon (0.5%, [w/v]) and nitrogen sources (0.5%, [w/v]). Therefore, it is feasible to use melanoidin on an industrial scale to decolorize distilled effluents.
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来源期刊
Journal of Pure and Applied Microbiology
Journal of Pure and Applied Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
2.00
自引率
0.00%
发文量
266
审稿时长
11 months
期刊介绍: Journal of Pure and Applied Microbiology (JPAM) is a peer-reviewed, open access international journal of microbiology aims to advance and disseminate research among scientists, academics, clinicians and microbiologists around the world. JPAM publishes high-quality research in all aspects of microbiology in both online and print form on quarterly basis.
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