利用鼻咽拭子进行 SARS-CoV-2 RT-PCR 检测 COVID-19 与 SARS-CoV-2 的快速抗原检测的性能评估,特别参考 RNA 依赖性 RNA 聚合酶 (RdRp) 周期阈值

IF 0.7 Q4 MICROBIOLOGY Journal of Pure and Applied Microbiology Pub Date : 2023-11-20 DOI:10.22207/jpam.17.4.28
Vidyut Prakash, Kumar Saurabh, Rishikesh Kumar, Randhir Kumar, N. Kumari, Shailesh Kumar
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引用次数: 0

摘要

SARS-CoV-2 具有高度传染性,即使没有临床症状的病人或仅有轻微症状的人也会传播。诊断该病毒的金标准检测方法是反转录 PCR(RT-PCR),但在病毒大流行时,需要进行快速抗原检测(RAT),这种检测的周转时间非常短。评估 COVID-19 快速抗原检测与使用鼻咽拭子进行的 SARS-CoV-2 RT-PCR 检测的性能比较,以及 RNA 依赖性 RNA 聚合酶(RdRp)周期阈值(Ct)。这项观察性横断面研究的对象是具有流感样病症(ILI)特征的患者、进行任何产生气溶胶的手术的患者或寻求住院治疗的高危患者。研究人员对每位患者的样本进行了 RT-PCR 和 RAT 检测,并对结果进行了比较。共检测了 5314 份样本,其中 104 份样本(01.95 %)和 229 份样本(04.31 %)在 RAT 和 RT PCR 检测中呈阳性。结果发现,RAT 的灵敏度、特异性、PPV 和 NPV 分别为 44.54%、99.96%、98.08% 和 97.56%。Ct值≤20的样本中,98.9%呈RAT阳性,而Ct值≥26的样本中,只有2.2%呈阳性。Ct 值越低的病例症状越明显,反之亦然。通过 RT-PCR 检测,RAT 不能有效检测出高 Ct 值(Ct ≥ 26)样本中的病毒。样本 Ct 值低(Ct ≤ 20)的患者症状更严重,反之亦然。
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Performance Evaluation of Rapid Antigen Test for COVID-19 with SARS-CoV-2 RT-PCR using Nasopharyngeal Swab with Special Reference to RNA Dependent RNA Polymerase (RdRp) Cycle Threshold Values
SARS-CoV-2 is highly contagious, which spreads even by patients having no clinical symptoms or also from people suffering with only mild symptoms. The gold standard test for its diagnosis is reverse-transcription PCR (RT-PCR) but at times of pandemic, Rapid antigen tests (RAT) are required, which has a very less turn-around time. Evaluation of the performance of COVID-19 Rapid antigen test in comparison to SARS-CoV-2 RT-PCR using nasopharyngeal swab, in relation to RNA dependent RNA polymerase (RdRp) Cycle threshold (Ct) values. This observational and cross-sectional study was done on patients coming with features of Influenza-like illness (ILI) or for any aerosol generating procedure or on high-risk patients seeking hospitalization. Both RT-PCR and RAT for COVID-19 were done on samples collected from each patient and results were compared. Altogether, 5314 samples were tested, out of which 104 (01.95 %) & 229 (04.31 %) samples were found positive by the RAT & RT PCR test, respectively. Sensitivity, specificity, PPV and NPV of RAT were found to be 44.54%, 99.96%, 98.08% and 97.56%, respectively. 98.9 % of samples with Ct value ≤ 20 were positive by RAT, whereas only 2.2% samples having Ct value ≥ 26 were found to be positive. Cases having lower Ct values were found to be more symptomatic and vice-versa. RAT are not efficient in detecting the virus in samples showing high Ct values (Ct ≥ 26) by RT-PCR test. Patients with samples showing low Ct values (Ct ≤ 20) had more severe symptoms and vice-versa.
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来源期刊
Journal of Pure and Applied Microbiology
Journal of Pure and Applied Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
2.00
自引率
0.00%
发文量
266
审稿时长
11 months
期刊介绍: Journal of Pure and Applied Microbiology (JPAM) is a peer-reviewed, open access international journal of microbiology aims to advance and disseminate research among scientists, academics, clinicians and microbiologists around the world. JPAM publishes high-quality research in all aspects of microbiology in both online and print form on quarterly basis.
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