珊瑚礁豆娘(Pomacentrus amboinensis)做出免疫反应的能量成本

IF 1 4区 生物学 Q3 ZOOLOGY Canadian Journal of Zoology Pub Date : 2023-11-15 DOI:10.1139/cjz-2023-0033
Marie Levet, Dominique G. Roche, S. Killen, Simona Colosio, R. Bshary, Joanna J. Miest, S. Binning
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引用次数: 0

摘要

虽然免疫反应可能会耗费能量,但量化这些成本却具有挑战性。我们测试了大豆娘(Pomacentrus amboinensis Bleeker, 1868)在注射大量脂多糖(LPS)内毒素后免疫激活的代谢成本。鱼类被分为八个处理:两个对照组(处理和注射生理盐水)和六个 LPS 组(浓度为 3 至 100 毫克/千克)。我们使用间歇流呼吸测定法测量注射 LPS 前 20 小时与注射后 20 小时的摄氧量(deltaṀO2)差异,以及代谢特征(最低、常规和峰值代谢率)的变化,作为有氧代谢成本的替代指标。测量了脾脏体细胞指数(SSI)和脾脏中的基因表达,以评估免疫激活情况。我们发现,暴露于 50 和 100mg kg-1 LPS 的鱼类与低剂量和对照组相比,代谢特征或 SSI 没有差异,但观察到δṀO2 的非线性模式不同。与对照组和低剂量 LPS 相比,暴露于高剂量 LPS 的鱼类的残余有氧范围也较低。与处理对照组相比,暴露于 3、50 和 100mg kg-1 剂量的鱼的基因表达发生了改变。总之,我们的研究结果表明,免疫激活对代谢特征具有可测量的影响,这种影响既与剂量有关,也与时间有关。
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Energetic costs of mounting an immune response in a coral reef damselfish (Pomacentrus amboinensis)
While immune responses can be energetically costly, quantifying these costs is challenging. We tested the metabolic costs of immune activation in damselfish (Pomacentrus amboinensis Bleeker, 1868) following a mass-adjusted injection of lipopolysaccharide (LPS) endotoxin. Fish were divided into eight treatments: two controls (handling and saline injection) and six LPS groups with concentrations ranging from 3 to 100mg kg-1. We used intermittent flow respirometry to measure differences in oxygen uptake (deltaṀO2) 20h before versus 20h after LPS injection and changes in metabolic traits (lowest, routine and peak metabolic rates) as proxies of the aerobic costs of metabolism. Spleen somatic index (SSI) and gene expression in spleens were measured to assess immune activation. We found no difference in metabolic traits or SSI but observed different non-linear patterns of deltaṀO2 in fish exposed to 50 and 100mg kg-1 LPS compared to lower doses and controls. Fish exposed to high doses of LPS also had lower residual aerobic scope compared to controls and lower LPS doses. Fish exposed to doses of 3, 50, and 100mg kg-1 showed altered gene expression compared to the handling control. Overall, our results suggest that immune activation has measurable effects on metabolic traits that are both dose and time-dependent.
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来源期刊
Canadian Journal of Zoology
Canadian Journal of Zoology 生物-动物学
CiteScore
2.40
自引率
0.00%
发文量
82
审稿时长
3 months
期刊介绍: Published since 1929, the Canadian Journal of Zoology is a monthly journal that reports on primary research contributed by respected international scientists in the broad field of zoology, including behaviour, biochemistry and physiology, developmental biology, ecology, genetics, morphology and ultrastructure, parasitology and pathology, and systematics and evolution. It also invites experts to submit review articles on topics of current interest.
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