银杏叶通过改善 Bcl-2/mTOR/ERK1/2/Na+、K+-ATPase 活性，对异丙肾上腺素诱发的心肌梗死具有多效减轻作用

IF 4.7 4区医学 Q1 CHEMISTRY, MEDICINAL Chinese Herbal Medicines Pub Date : 2024-04-01 DOI:10.1016/j.chmed.2023.11.001

Jerome Ndudi Asiwe , Abodunrin Adebayo Ojetola , Nwoke Enekabokom Ekene , Esthinsheen Osirim , Anthony Chibuzor Nnamudi , Benjamin Oritsemuelebi , Jackson Erozueme Onuelu , Nicholas Asiwe , Harrison Ogheneochuko Eruotor , Saviour Inegbenehi

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引用次数: 0

摘要

目标心肌梗死（MI）与心肌血氧供需失衡有关。β-受体阻滞剂和钙拮抗剂是治疗心肌梗塞的两种常用药物。但据报道，这些药物要么无效，要么有不良副作用。银杏叶提取物（GBE）是一种中草药产品，在治疗炎症性疾病和氧化应激方面具有特殊的兼容性优势。为了更好地了解 GBE 如何影响异丙肾上腺素（ISO）损伤大鼠的心肌梗死，本研究进行了设计。方法心脏重量指数、血清脂质概况、心脏标志酶、内源性抗氧化剂[过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、亚硝酸盐和丙二醛（MDA）]、炎症介质[肿瘤坏死因子α（TNF-α）和白细胞介素-6（IL-6）]、采用 B 细胞淋巴瘤因子-2（Bcl-2）、细胞外信号调节激酶（ERK1/2）和哺乳动物雷帕霉素靶标（mTOR）的免疫组化表达以及组织病理学分析来评估 GBE 的心脏保护特性。结果研究结果表明，GBE能增强机体的天然抗氧化防御系统，减少炎性细胞因子和心脏损伤标志酶的释放，从而有效减轻心肌梗死。结论GBE对心脏的保护作用可能是由于其增加了Bcl-2/mTOR/ERK1/2/Na+、K+-ATPase活性的机制。

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Pleiotropic attenuating effect of Ginkgo biloba against isoprenaline-induced myocardial infarction via improving Bcl-2/mTOR/ERK1/2/Na+, K+-ATPase activities

Objective

Myocardial infarction (MI) is linked to an imbalance in the supply and demand of blood oxygen in the heart muscles. Beta-blockers and calcium antagonists are just two of the common medications used to treat MI. However, these have reportedly been shown to be either ineffective or to have undesirable side effects. Extract of Ginkgo biloba leaves (GBE), a Chinese herbal product offers special compatibility benefits in therapeutic settings relating to inflammatory diseases and oxidative stress. In order to better understand how GBE affects MI in rats insulted by isoprenaline (ISO), the current study was designed.

Methods

The heart weight index, serum lipid profile, cardiac marker enzymes, endogenous antioxidants [catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), nitrites and malondialdehyde (MDA)], inflammatory mediators [tumour necrosis factor alpha (TNF-α) and interleukin-6 (IL-6)], immunohistochemical expressions of B-cell lymphoma factor-2 (Bcl-2), extracellular signal-regulated kinase (ERK1/2), and mammalian target of rapamycin (mTOR) and histopathological analysis were used to assess the cardioprotective properties of GBE.

Results

The findings showed that GBE effectively attenuated myocardial infarction by boosting the body’s natural antioxidant defense system and reducing the release of inflammatory cytokines as well as heart injury marker enzymes. The expression of Bcl-2, ERK1/2 and mTOR was increased while the histomorphological alterations were reversed.