鉴定菠萝种植园中的本地钾溶解细菌

Kusuma Handayani, Mirawanty Amin
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引用次数: 0

摘要

在种植园规模的菠萝(Ananas comosus)种植中,使用相对高剂量的无机肥料来提高产量可能会导致新的问题,如对植物有毒、污染环境和增加生产成本。利用根瘤微生物,如钾溶解菌(KSB),有望减少无机钾肥的施用量,因为它能溶解土壤中固定的钾铝和钾铁,从而使植物可以利用。本研究旨在评估和描述菠萝种植区中 BPK 的潜力。根据钾溶解度指数(IKK)对 KSB 进行了评估,此外还对有潜力成为 CPC 分离物的细菌进行了鉴定。从无性期(V)和生长期(G)产量水平较低和较高的种植区块中分离出了 CPC,共进行了 5 次重复。在这项研究中,从菠萝种植园的 CPC 群体中成功分离出了多达 20 个分离株。分离物 RV1 的 K 溶解指数最低,为 0.6,分离物 RG1 的 K 溶解指数最高,为 1.2。在这些分离物中,RG1 是革兰氏阳性菌,在 pH 值为 5、温度为 30°C 的条件下生长最佳。根据系统进化分析的结果可知,该钾溶解分离物与枯草芽孢杆菌具有相同的相似性。 关键词:枯草芽孢杆菌枯草芽孢杆菌 本地钾 增溶指数 温度
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Identifikasi Bakteri Pelarut Kalium Indigenus Asal Perkebunan Nanas
The use of relatively high doses of inorganic fertilizers to increase yields in plantation-scale pineapple (Ananas comosus) cultivation can lead to new problems, such as being toxic to plants, polluting the environment, and increasing production costs. The utilization of rhizosphere microbes, such as potassium solubilizing bacteria (KSB) is expected can reduce the dose of inorganic K fertilizer application because it can dissolve potassium-fixed Al and Fe soil so that it is available to plants. This study aims to assess and characterize the potential of BPK in pineapple plantation areas. KSB evaluation was carried out based on the Potassium Solubility Index (IKK), besides that, it also carried out the characterization of bacteria that have the potential as CPC isolates. CPC was isolated from planting blocks with low and high production levels in the vegetative (V) and generative (G) phases with 5 replications. As many as 20 isolates from the CPC population on pineapple plantations were successfully isolated in this study. The lowest K solubility index of 0.6 was obtained in isolate RV1 and the highest, namely 1.2 in isolate RG1. Among these isolates, RG1 is Gram-positive and grows optimally at pH 5, temperature 30°C. Based on the results of the phylogenetic analysis, it is known that this potassium solubilizing isolate has the same level of similarity with the bacterium Bacillus subtilis.   Keywords: Bacillus, indigenous, potassium, solubilizing index, temperature
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