用各种寄主植物繁殖希雷迈山国家公园的本地菌根

Ai Nurlaila, Ika Karyaningsih, Dede Kosasih, Ilham Adhya, Meindhika Giwantara, Wiwit Walinda
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摘要

本研究旨在确定使用寄主植物繁殖古农吉赖迈国家公园本地菌根繁殖体的有效性。实验方法采用了包含两个因素的因子完全随机设计法。第一个因素是接种源的位置,分为 3 个等级:混交林、灌木林和松林。第二个因素是 4 种寄主植物,分别为玉米(Zea mays)、高粱(Sorghum bicolor. (L.) Moench)、菜豆(Centrosema pubescens)和芸豆(Pueraria javanica)。每个处理重复五次,因此共有 60 个实验单位。孢子分离采用湿过滤和倾析法,该方法借鉴了 Gerdemann 和 Nicolson(1963 年)的方法,然后采用 Jenkins(1964 年)的改良糖离心法。观察参数是菌根孢子的数量。所得数据通过方差检验(F 检验)进行分析。为了评估处理效果并比较所选处理,采用了 5%水平的邓肯后续检验。灌木接种体位置和中心豆寄主植物的组合显示出最大的孢子数量,每 50 克土壤样本中的孢子数量为 222.60 个。使用玉米、高粱、四季豆和芸豆等寄主植物进行诱捕的结果并不理想。最常见的菌属是 Glomus(80%;4 种)、Gigaspora(10%,1 种)和 Acaulospora(10%,1 种)。在所有诱捕结果中都均匀地发现了 Glomus。 关键词:AcaulosporaAcaulospora;Glomus;菌根;繁殖体;孢子
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Perbanyakan Mikoriza Indigenus Taman Nasional Gunung Ciremai dengan Berbagai Tanaman Inang
This study aimed to determine the effectiveness of the use of host plants in an effort to multiply mycorrhizal propagules indigenous to Gunung Ciremai National Park. The experimental method used a factorial complete randomized design with 2 factors. The first factor was the location of the source of inoculum by 3 levels: mixed forests, shrubs, and pine stands. The second factor was 4 types of host plant many as 4 levels: corn (Zea mays), sorghum (Sorghum bicolor. (L.) Moench), centro beans (Centrosema pubescens), and ruji beans (Pueraria javanica). Each treatment was repeated five times, so there were 60 experimental units. The spores were isolated by wet filtration and decantation methods adapted from Gerdemann and Nicolson (1963), followed by the modified sugar centrifugation method from Jenkins (1964). The observed parameter was the number of mycorrhizal spores. The data obtained were analyzed by a variance test (F test). To evaluate the treatment's effect and compare the selected treatments, Duncan's follow-up test was used at the level of 5%. The combination of the location of the shrub inoculum and the centro bean host plant showed the largest spore population, which was 222.60 per 50 g soil sample. Trapping techniques using host plants of corn, sorghum, centro beans, and ruji beans showed suboptimal results. The most common genera were Glomus (80%; 4 species), Gigaspora (10%, 1 species), and Acaulospora (10%, 1 species). Glomus was evenly found in all trapping results.   Keywords: Acaulospora, Glomus, mycorrhiza, propagules, spores
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