Production of Sea Buckthorn Oil by Extraction of Sea Buckthorn Fruit Pulp with Supercritical Carbon Dioxide Followed by Centrifugation

A simple method for production of buckthorn buckthorn oil (Hippophae rhamnoides) with a total carotenoid content sufficient to meet the pharmacopoeial requirements for sea buckthorn oil used as a wound healing agent is proposed. The method is based on the extraction of dry pulp of sea buckthorn fruit with supercritical carbon dioxide at high pressures (50 MPa) to achieve a high content of carotenoids in the oil, followed by centrifugation to separate ballast solid wax fractions. Chromatographic and photometric analysis of the chemical composition of the oil confirm the prospects of this approach to obtaining sea buckthorn oil.