实现葡萄零杀菌剂残留的管理策略

K. T. Suryawanshi, S. D. Sawant, I. Sawant
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摘要

研究人员尝试在葡萄种植过程中,在必要时使用杀虫剂,并保证施用时间和杀虫剂的选择,以便在收获时尽可能减少残留。在坐果前和坐果后,防治病害的策略有所不同。为了防治霜霉病,使用了二硫代氨基甲酸酯类药物,如 mancozeb,因此无法使用生物防治剂进行喷洒。为了达到最高的 ISR 活性,在果实修剪前和修剪后立即使用毛霉。多年来,葡萄白粉病的防治主要使用试唑类杀菌剂。但近年来,葡萄白粉病对三唑类杀菌剂的抗药性增加到 50 ppm。因此,从战略上讲,决定在果实修剪后 60 天不再使用三唑类杀菌剂。硫磺是更安全的替代品,这是一个显而易见的选择,这意味着如果只喷洒硫磺,也可以使用生物防治剂。在所有 FLD 地点,修剪后 60 天内只使用硫磺和生物防治剂,如毛霉和霜霉病菌,并交替使用,直到收获前 15 天左右。根据生物药效实验,二氧化氯、纳米银与过氧化氢的复合物被选为用于防治白粉病的生态低影响化学品。在所有四个 FLD 地块上都实施了这一策略,所有 FLD 地块上霜霉病、白粉病和炭疽病的病害 PDI 都保持在最低水平。葡萄园中农药残留的消散可能取决于许多因素,微生物降解就是其中一个重要因素。ICAR-NRCG 开发了名为 DR-39 的枯草芽孢杆菌,并进行了商业配制,有效剂量为 2.5 克/升,可提高约 10 种杀虫剂的消散率。2017-18年期间,在不同FLD的葡萄中检测到的杀菌剂总残留量在0-3之间。所有检测到的杀菌剂均低于最高残留限量(MRL),其浓度在十亿分之一(ppb)范围内非常低。在零残留地块种植的葡萄,其货架期、生理失重率(PLW)和烂果率均低于农民种植地块。通过使用最新的技术和策略,FLDs 成功地种植出了没有检测到任何农药残留的葡萄(零残留葡萄)。
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Management Strategies for Zero Fungicides Residue in Grapes
Research was attempted for growing grapes by using pesticides whenever obligatory and keeping time of applications and choice of pesticides such that at harvest residue will be minimum possible. Strategically outlook for control of diseases was different before fruit set and thereafter. For the management of downy mildew, dithiocarbamate group, such as mancozeb are used, due to which use of bio-control agents as spray was not possible. Induce systemic resistance (ISR) activities of microbes was exploit, to achieve highest ISR activities use of Trichoderma was given before and instantly after fruit pruning. For years, grapes powdery mildew management is dominated by use of trialzole fungicides. But in recent year resistance to triazole fungicides in powdery mildew accentuated up to 50 ppm. Therefore, strategically it was decided that triazole fungicides will not be used 60 days after fruit pruning. The sulfur was safer substitute, an obvious choice which meant if only sulfur was sprayed bio-control agents also can use. Only sulfur and bio-control agents such as Trichoderma and Ampelomyces were used in all FLD locations 60 days after pruning with alternation, till about 15 days before harvest. Based on bio-efficacy experiments chlorine dioxide, nano silver in complex with hydrogen peroxide were selected as ecologically low profile chemicals for management of powdery mildew. In all four FLD plots on this strategy was implemented and in all FLD plots disease PDI for downy mildew, powdery mildew and anthracnose were kept to be bare minimum. Dissipation of pesticide residue in vineyards may be dependent on many factors; microbial degradation is one of such important factors. ICAR-NRCG has developed and commercially formulated Bacillus subtilis name DR-39 applied at 2.5 g/L as effective dose to increase the rate dissipation of about 10 pesticides. Total fungicide residues detected in grapes from different FLD, during 2017-18 was in the range of 0-3. All fungicides detected were below MRLs (Maximum Residue Limits) and their concentrations were very less in parts per billion (ppb) range. The shelf life of the grapes grown in zero residue plots, per cent physiological weight loss (PLW) and per cent rotten berries were found less as compared to farmers practiced plot. The FLDs have success for growing grapes without any detectable pesticides residue (zero residue grapes) by using updated technology and strategy.
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