在小鼠和人类细胞系中,染色质重塑复合物 PBAF 的 PHF10D 亚基的表达伴随着增殖的接触抑制。

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Doklady Biochemistry and Biophysics Pub Date : 2024-01-08 DOI:10.1134/S1607672923700667
Yu. P. Simonov, V. V. Tatarskiy,  S. G. Georgieva, N. V. Soshnikova
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引用次数: 0

摘要

PHF10 是 PBAF 复合物的一个亚基,PBAF 复合物调节发育和成熟生物体中许多基因的表达。PHF10 有四种异构体,它们的结构域各不相同。PHF10A 异构体的 C 端含有 DPF 结构域,N 端含有 46 个氨基酸,是增殖基因表达所必需的;其他异构体的功能研究较少。在这项工作中,我们证实了当细胞之间建立紧密连接和粘附连接而导致小鼠和人类细胞增殖受到接触性抑制时,PHF10A 同工酶的表达会停止,取而代之的是不含 DPF 结构域和 N 端序列的 PHF10D 同工酶的表达。PHF10D 异构体的功能可能与细胞间接触的建立有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Contact Inhibition of Proliferation Is Accompanied by Expression of the PHF10D Subunit of the Chromatin Remodeling Complex PBAF in Mouse and Human Cell Lines

PHF10 is a subunit of the PBAF complex, which regulates the expression of many genes in developing and maturing organisms. PHF10 has four isoforms that differ in domain structure. The PHF10A isoform, containing a DPF domain at the C-terminus and 46 amino acids at the N-terminus, is necessary for the expression of proliferation genes; the functions of the other isoforms are less studied. In this work, we have established that, upon contact inhibition of mouse and human cell proliferation caused by the establishment of a tight junction and adherence junction between cells, the expression of the PHF10A isoform stops and instead the PHF10D isoform is expressed, which does not contain DPF-domain and N-terminal sequence. The function of the PHF10D isoform may be associated with the establishment of intercellular contacts.

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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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