精选精油对牛乳腺炎金黄色葡萄球菌的抗菌活性

Dairy Pub Date : 2024-01-05 DOI:10.3390/dairy5010005
Karen Vanessa Munive Nuñez, Anderson Clayton da Silva Abreu, Jaqueline Milagres de Almeida, J. L. Gonçalves, É. C. Bonsaglia, M. V. dos Santos, Nathália Cristina Cirone Silva
{"title":"精选精油对牛乳腺炎金黄色葡萄球菌的抗菌活性","authors":"Karen Vanessa Munive Nuñez, Anderson Clayton da Silva Abreu, Jaqueline Milagres de Almeida, J. L. Gonçalves, É. C. Bonsaglia, M. V. dos Santos, Nathália Cristina Cirone Silva","doi":"10.3390/dairy5010005","DOIUrl":null,"url":null,"abstract":"Staphylococcus aureus is a major cause of subclinical mastitis in dairy cows, and its development of antibiotic resistance has limited treatment efficacy. Essential oils (EOs) are natural products with a wide range of antimicrobial properties that could be used to treat bovine mastitis. This study aims to investigate the antimicrobial activity of EOs against S. aureus isolated from subclinical bovine mastitis cases in the State of São Paulo—Brazil. A total of 14 S. aureus isolates were selected, based on the presence of biofilm-forming genes (icaA, icaD, and bap), and were cultured to a final concentration of 103 CFU.mL−1 for the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) analysis of five EOs (Citrus aurantium bergamia—bergamot, Copaifera reticulata—copaiba, Foeniculum vulgare—fennel, Zingiber officinale—ginger, and Ocimum basilicum—basil). The chemical compositions of the EOs were characterized using gas chromatography coupled with a mass-selective detector (GC/MSD). Basil and bergamot EOs exhibited the highest antimicrobial activity against S. aureus strains, with mean MIC/MBC values of 1.561 ± 0.223/2.806 ± 0.255 mg.mL−1 and 2.782 ± 0.228/4.396 ± 0.198 mg.mL−1, respectively. The primary compounds in basil EO were methyl-chavicol, linalool, and α-humulene, while bergamot EO predominantly contained linalyl acetate, limonene, and linalool. This research highlights the potential of basil and bergamot EOs as natural antimicrobial agents for treating bovine mastitis caused by S. aureus, offering a potential alternative to traditional antibiotics and contributing to animal welfare and public health. In addition, it emphasizes the need for further studies to validate the long-term effects, optimal dosages, and application methods.","PeriodicalId":505869,"journal":{"name":"Dairy","volume":"22 3","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antimicrobial Activity of Selected Essential Oils against Staphylococcus aureus from Bovine Mastitis\",\"authors\":\"Karen Vanessa Munive Nuñez, Anderson Clayton da Silva Abreu, Jaqueline Milagres de Almeida, J. L. Gonçalves, É. C. Bonsaglia, M. V. dos Santos, Nathália Cristina Cirone Silva\",\"doi\":\"10.3390/dairy5010005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Staphylococcus aureus is a major cause of subclinical mastitis in dairy cows, and its development of antibiotic resistance has limited treatment efficacy. Essential oils (EOs) are natural products with a wide range of antimicrobial properties that could be used to treat bovine mastitis. This study aims to investigate the antimicrobial activity of EOs against S. aureus isolated from subclinical bovine mastitis cases in the State of São Paulo—Brazil. A total of 14 S. aureus isolates were selected, based on the presence of biofilm-forming genes (icaA, icaD, and bap), and were cultured to a final concentration of 103 CFU.mL−1 for the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) analysis of five EOs (Citrus aurantium bergamia—bergamot, Copaifera reticulata—copaiba, Foeniculum vulgare—fennel, Zingiber officinale—ginger, and Ocimum basilicum—basil). The chemical compositions of the EOs were characterized using gas chromatography coupled with a mass-selective detector (GC/MSD). Basil and bergamot EOs exhibited the highest antimicrobial activity against S. aureus strains, with mean MIC/MBC values of 1.561 ± 0.223/2.806 ± 0.255 mg.mL−1 and 2.782 ± 0.228/4.396 ± 0.198 mg.mL−1, respectively. The primary compounds in basil EO were methyl-chavicol, linalool, and α-humulene, while bergamot EO predominantly contained linalyl acetate, limonene, and linalool. This research highlights the potential of basil and bergamot EOs as natural antimicrobial agents for treating bovine mastitis caused by S. aureus, offering a potential alternative to traditional antibiotics and contributing to animal welfare and public health. In addition, it emphasizes the need for further studies to validate the long-term effects, optimal dosages, and application methods.\",\"PeriodicalId\":505869,\"journal\":{\"name\":\"Dairy\",\"volume\":\"22 3\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-01-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Dairy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/dairy5010005\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dairy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/dairy5010005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

金黄色葡萄球菌是奶牛亚临床乳腺炎的主要病因,它对抗生素产生的抗药性限制了治疗效果。精油(EO)是具有多种抗菌特性的天然产品,可用于治疗牛乳腺炎。本研究旨在调查从巴西圣保罗州亚临床牛乳腺炎病例中分离出的金黄色葡萄球菌对 EO 的抗菌活性。根据生物膜形成基因(icaA、icaD 和 bap)的存在情况,共筛选出 14 个金黄色葡萄球菌分离株,并将其培养至 103 CFU.mL-1 的最低抑菌浓度 (MIC) 和最低杀菌浓度 (MBC)。使用气相色谱-质量选择检测器(GC/MSD)对茴香油的化学成分进行了鉴定。罗勒和佛手柑环氧乙烷对金黄色葡萄球菌菌株的抗菌活性最高,平均 MIC/MBC 值分别为 1.561 ± 0.223/2.806 ± 0.255 mg.mL-1 和 2.782 ± 0.228/4.396 ± 0.198 mg.mL-1。罗勒环氧乙烷中的主要化合物是甲基辣椒素、芳樟醇和 α-胡麻烯,而佛手柑环氧乙烷则主要含有乙酸芳樟酯、柠檬烯和芳樟醇。这项研究强调了罗勒和佛手柑环氧乙烷作为天然抗菌剂治疗金黄色葡萄球菌引起的牛乳腺炎的潜力,为传统抗生素提供了潜在的替代品,有助于动物福利和公共卫生。此外,它还强调了进一步研究的必要性,以验证其长期效果、最佳剂量和应用方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Antimicrobial Activity of Selected Essential Oils against Staphylococcus aureus from Bovine Mastitis
Staphylococcus aureus is a major cause of subclinical mastitis in dairy cows, and its development of antibiotic resistance has limited treatment efficacy. Essential oils (EOs) are natural products with a wide range of antimicrobial properties that could be used to treat bovine mastitis. This study aims to investigate the antimicrobial activity of EOs against S. aureus isolated from subclinical bovine mastitis cases in the State of São Paulo—Brazil. A total of 14 S. aureus isolates were selected, based on the presence of biofilm-forming genes (icaA, icaD, and bap), and were cultured to a final concentration of 103 CFU.mL−1 for the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) analysis of five EOs (Citrus aurantium bergamia—bergamot, Copaifera reticulata—copaiba, Foeniculum vulgare—fennel, Zingiber officinale—ginger, and Ocimum basilicum—basil). The chemical compositions of the EOs were characterized using gas chromatography coupled with a mass-selective detector (GC/MSD). Basil and bergamot EOs exhibited the highest antimicrobial activity against S. aureus strains, with mean MIC/MBC values of 1.561 ± 0.223/2.806 ± 0.255 mg.mL−1 and 2.782 ± 0.228/4.396 ± 0.198 mg.mL−1, respectively. The primary compounds in basil EO were methyl-chavicol, linalool, and α-humulene, while bergamot EO predominantly contained linalyl acetate, limonene, and linalool. This research highlights the potential of basil and bergamot EOs as natural antimicrobial agents for treating bovine mastitis caused by S. aureus, offering a potential alternative to traditional antibiotics and contributing to animal welfare and public health. In addition, it emphasizes the need for further studies to validate the long-term effects, optimal dosages, and application methods.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.40
自引率
0.00%
发文量
0
期刊最新文献
Morphokinetics of In Vitro-Derived Embryos—A Lesson from Human and Bovine Studies The Short-Term Effects of Altering Milking Intervals on Milk Production and Behavior of Holsteins Milked in an Automated Milking System Milk Quality and Economic Sustainability in Dairy Farming: A Systematic Review of Performance Indicators Effect of Yogurt on the Deodorization of Raw Garlic (Allium sativum L.) Sulfur Volatiles in Breath and the Roles of Its Components Influence of Deliverable Form of Dietary Vitamin D3 on the Immune Response in Late-Lactating Dairy Goats
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1