苏云金芽孢杆菌生物杀虫剂对胡萝卜胚胎期节肢动物含量的评估

Novian Dwisatria, Y. Pujiastuti, C. Irsan, Fitri Ramadhani
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摘要

农民仍在广泛使用合成杀虫剂来控制胡萝卜中的害虫。过量使用合成杀虫剂会破坏农业生态系统。这项研究旨在考察苏云金芽孢杆菌生物杀虫剂对胡萝卜无性生长期节肢动物的影响。所研究的胡萝卜种植园与芥菜和甜玉米进行了多元栽培。本研究采用完全随机设计(CRD),3 个处理 9 次重复。处理包括生物杀虫剂苏云金杆菌、合成杀虫剂(吡虫啉 200 克/升)和不施用杀虫剂(对照)。农艺观察包括胡萝卜的高度和叶片数。节肢动物观察采用了扫网、坑式诱捕器和直接肉眼观察法。结果表明,三种施药方法对胡萝卜株高和叶片数的影响无明显差异。施用苏云金杆菌的胡萝卜植株中节肢动物的数量低于对照胡萝卜植株。在坑式诱捕器观察中,膜翅目节肢动物个体数量最多,香农-维纳多样性指数(H')值适中。此外,使用诱捕网观察施用苏云金杆菌后的昆虫总数,从第二次观察开始呈下降趋势。在苏云金杆菌生物杀虫剂处理中,坑式诱捕器诱捕的昆虫多样性水平属于中等类别(H' = 1.75),而吡虫啉处理(H' = 0.85)和对照(H' = 0.81)属于低类别。
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EVALUATION OF Bacillus thuringiensis-BASED BIOINSECTICIDE ON THE PRESENCE OF ARTHROPODS IN VEGETATIVE PHASE OF CARROT
Synthetic insecticides are still extensively used by farmers to control insect pests in carrots. The effects of excessive use of synthetic insecticides can damage agricultural ecosystems. This research aimed to examine Bacillus thuringiensis based bioinsecticide toward arthropods existence in vegetative growth phase of carrots The research was conducted in Pagar Alam City (700 m above sea level) and a relative humidity of 48–99%. The carrot plantations studied were planted in polyculture with mustard greens and sweet corn. This study used a completely randomized design (CRD) with 3 treatments and 9 replications. The treatments were bioinsecticide B. thuringiensis; synthetic insecticide (imidacloprid 200 g/l); and no-insecticide application (control). Agronomic observations were height of carrot and their number of leaves. Arthropods observations were carried out using sweep nets, pitfall traps, and direct visual observation. The results showed there was no significantly different on height of carrot plant and their number of leaves among three applications. Arthropods population in carrot plants treated with B. thuringiensis was lower than those in control carrot plants. In pitfall trap observations, the highest number of arthropod individuals obtained was belong to order Hymenoptera and had a moderate value of the Shannon-Wiener diversity index (H'). In addition, total insect population after application of B. thuringiensis observed using nets, tended to decrease from the second observation onwards. The category of insect diversity level trapped by Pitfall trap in B. thuringiensis bioinsecticide treatment was included in the medium category (H' = 1.75), while the treatment of imidacloprid (H' = 0.85) and control (H' = 0.81) was included in the low category.
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