墨西哥北部零售新鲜农产品中出现的食源性病毒和体细胞噬菌体。

IF 4.1 2区 农林科学 Q2 ENVIRONMENTAL SCIENCES Food and Environmental Virology Pub Date : 2024-01-10 DOI:10.1007/s12560-023-09578-9
Axel Ossio, Fernanda Flores-Rodríguez, Norma Heredia, Santos García, Jose Angel Merino-Mascorro
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引用次数: 0

摘要

与食用蔬菜有关的食源性疾病暴发通常归因于人类肠道病毒,如诺如病毒(NoV)、甲型肝炎病毒(HAV)和轮状病毒(RoV)。在许多生鲜生产国,有关这些病毒发生情况的信息很少。病毒污染检测指标(如体细胞大肠杆菌)可间接反映病毒病原体的存在,是食品行业更好地进行病毒风险评估的重要工具。本研究旨在确定墨西哥北部叶菜中食源性病毒和体细胞噬菌体的发生率和相关性。共收集了 320 个蔬菜样本,其中 80 个为复合漂洗样本,40 个为莴苣样本,40 个为欧芹样本。采用 EPA 1602 方法对体细胞大肠杆菌进行了测定,同时采用 qPCR 方法对食源性病毒(HAV、RoV、NoV GI 和 GII)进行了测定。莴苣中 RoV 的发生率为 22.5%(9/40,平均 2.11 log gc/g),欧芹中为 20%(8/40,平均 1.91 log gc/g)。所有样本中均未检测到 NoV 和 HAV。所有生菜和欧芹样本中都存在体细胞大肠杆菌,平均水平分别为 1.85 log PFU/100 ml 和 2.28 log PFU/100ml。斯皮尔曼分析确定了体细胞噬菌体与 RoV 基因组拷贝的相关性,结果是生菜中的 r2 值为 - 0.026,西芹中的 r2 值为 0.349。虽然样本中未检测到 NoV 或 HAV,但 RoV 的存在令人担忧,因为绿叶菜通常生吃,有潜在的感染风险。
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Foodborne Viruses and Somatic Coliphages Occurrence in Fresh Produce at Retail from Northern Mexico

Foodborne disease outbreaks linked to consumption of vegetables have been often attributed to human enteric viruses, such as Norovirus (NoV), Hepatitis A virus (HAV), and Rotavirus (RoV). Information about the occurrence of these viruses is scarce in many fresh-producing countries. Viral contamination detection of indicators, such as somatic coliphages, could indirectly reflect the presence of viral pathogens, being a valuable tool for better viral risk assessment in food industry. This study aimed to establish the occurrence and correlation of foodborne viruses and somatic coliphages in leafy greens in northern Mexico. A total of 320 vegetable samples were collected, resulting in 80 composite rinses, 40 of lettuce and 40 of parsley. Somatic coliphages were determined using the EPA 1602 method, while foodborne viruses (HAV, RoV, NoV GI, and GII) were determined by qPCR. The occurrence of RoV was 22.5% (9/40, mean 2.11 log gc/g) in lettuce and 20% (8/40, mean 1.91 log gc/g) in parsley. NoV and HAV were not detected in any samples. Somatic coliphages were present in all lettuce and parsley samples, with mean levels of 1.85 log PFU/100 ml and 2.28 log PFU/100 ml, respectively. Spearman analysis established the correlation of somatic coliphages and genomic copies of RoV, resulting in an r2 value of − 0.026 in lettuce and 0.349 in parsley. Although NoV or HAV were undetected in the samples, the presence of RoV is a matter of concern as leafy greens are usually eaten raw, which poses a potential risk of infection.

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来源期刊
Food and Environmental Virology
Food and Environmental Virology ENVIRONMENTAL SCIENCES-MICROBIOLOGY
CiteScore
6.50
自引率
2.90%
发文量
35
审稿时长
1 months
期刊介绍: Food and Environmental Virology publishes original articles, notes and review articles on any aspect relating to the transmission of pathogenic viruses via the environment (water, air, soil etc.) and foods. This includes epidemiological studies, identification of novel or emerging pathogens, methods of analysis or characterisation, studies on survival and elimination, and development of procedural controls for industrial processes, e.g. HACCP plans. The journal will cover all aspects of this important area, and encompass studies on any human, animal, and plant pathogenic virus which is capable of transmission via the environment or food.
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