人脂肪干细胞在两阶段家兔骨关节炎模型中上调 IGF-1 并缓解骨关节炎。

Juan Wang, Shibo Su, Chuanming Dong, Qiang Fan, Jishu Sun, Siqiang Liang, Zuhuo Qin, Chuqing Ma, Jianfeng Jin, Hongwen Zhu, Tongmeng Jiang, Jun Xu
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摘要

目的:近年来,人们已经知道间充质干细胞(MSCs)具有治疗骨关节炎(OA)的潜力。本研究旨在探讨在新的双重损伤兔骨关节炎模型中关节内注射人脂肪来源干细胞(hADSCs)的效果:首先通过内侧副韧带、前插入韧带横断和内科半月板切除术建立OA模型,然后进行关节软骨全厚缺损。术后六周,用表达慢病毒 FG12 的增强型绿色荧光蛋白标记 hADSCs 并将其注入膝关节。所有兔子分别于术后4周和8周处死。评估方法包括宏观检查、免疫组化染色、磁共振成像、qRT-PCR 和 ELISA 分析:结果:注射 hADSCs 4 周和 8 周后,软骨损失较少,裂缝和裂纹较少,关节积液量减少,核磁共振成像显示软骨缺损。此外,ELISA和qRT-PCR方法显示,hADSCs治疗可提高IGF-1的水平:我们的数据表明,hADSCs移植可促进双重损伤兔骨关节炎模型的关节软骨愈合,IGF-1可能在以hADSCs为基础的软骨修复过程中发挥了重要作用。hADSCs移植可能适合用于骨关节炎的临床治疗。
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Human Adipose-derived Stem Cells Upregulate IGF-1 and Alleviate Osteoarthritis in a Two-stage Rabbit Osteoarthritis Model.

Objective: In recent times, it has been recognized that mesenchymal stem cells (MSCs) possess the capability to address osteoarthritis (OA). The objective of this research was to examine the impact of injecting human adipose-derived stem cells (hADSCs) into a novel rabbit osteoarthritis model with dual damage.

Methods: The OA model was established surgically first by medial collateral ligament and anterior cruciate ligament transection and medial meniscectomy, then by articular cartilage full-thickness defect. Enhanced Green Fluorescence Protein expressing lentivirus FG12 was used to label hADSCs, which were then injected into the knee joints. Every single rabbit was sacrificed after 4 and 8 weeks following the surgical procedure. Macroscopic examination, immunohistochemistry staining, magnetic resonance imaging, qRT-PCR, and ELISA analysis were utilized for the assessments.

Results: After 4 and 8 weeks, the injection of hADSCs resulted in reduced cartilage loss, minimal fissures and cracks, and a decrease in the volume of joint effusion and cartilage defect as measured by MRI. Moreover, the application of ELISA and qRT-PCR techniques revealed that the administration of hADSCs resulted in an elevation in the IGF-1 concentration.

Conclusions: Based on our findings, it can be inferred that the transplantation of hADSCs facilitates the healing of articular cartilage in the osteoarthritis model of rabbits with double damage. The upregulated IGF-1 may play a crucial part in the process of cartilage repair using hADSCs. The use of hADSC transplantation could potentially be appropriate for clinical implementation in managing osteoarthritis.

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