Fatima Hoosen, Jordan Tonga Lembe, Derek Ndinteh, Tagatsing Fotsing Maurice, Yankep Emmanuel, Lesetja Raymond Motadi, Mpho Susan Choene
{"title":"从 Millettia griffoniana 中提取的活性化合物对胰腺癌和结直肠癌细胞的抗癌潜力","authors":"Fatima Hoosen, Jordan Tonga Lembe, Derek Ndinteh, Tagatsing Fotsing Maurice, Yankep Emmanuel, Lesetja Raymond Motadi, Mpho Susan Choene","doi":"10.1007/s13596-023-00733-y","DOIUrl":null,"url":null,"abstract":"<div><p>Pancreatic and colorectal cancer are two of the most lethal cancers; this stems from poor prognosis. Current treatments may lack effectiveness and produce dangerous side effects, hence a need for alternatives. Medicinal plants like <i>Millettia griffoniana</i> may possess anti-cancer properties. This study aimed to isolate and identify compounds from <i>M. griffoniana</i> to test for potential anti-cancer activity on pancreatic and colorectal cancer. Seeds and root bark of <i>M. griffoniana</i> underwent solvent extraction and separation using column chromatography. Isolated compounds were screened using AlamarBlue assays and cytotoxic compounds were identified with 2D NMR. Compounds were subject to cellular viability assays using AlamarBlue and xCELLigence analysis. The Caspase Glo®-3/7 kit measured caspase activity and Real-Time PCR analysis measured apoptosis-related gene expression. 7 compounds were isolated and screened. Compounds 5 and 7 were chosen; identified as durmillone and isojamaicin. Both showed varying concentration-dependent cytotoxic activity, in AlamarBlue and xCELLigence assays, for both cell lines. Caspases 3 and 7 were up-regulated and both compounds up-regulated <i>BAX</i> and down-regulated <i>BCL-2 a</i>nd <i>p53</i> in both cell lines. Durmillone and isojamaicin displayed cytotoxic activity on pancreatic and colorectal cancer. Apoptotic activity induced by the compounds was verified by the up-regulation of caspase activity and <i>BAX</i> and down-regulation of <i>BCL-2</i> and <i>p53</i>. Further studies need to be done towards understanding the mechanisms by which these compounds bring about their cytotoxic activity.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-023-00733-y.pdf","citationCount":"0","resultStr":"{\"title\":\"The anti-cancer potential of active compounds extracted from Millettia griffoniana on pancreatic and colorectal cancer cells\",\"authors\":\"Fatima Hoosen, Jordan Tonga Lembe, Derek Ndinteh, Tagatsing Fotsing Maurice, Yankep Emmanuel, Lesetja Raymond Motadi, Mpho Susan Choene\",\"doi\":\"10.1007/s13596-023-00733-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Pancreatic and colorectal cancer are two of the most lethal cancers; this stems from poor prognosis. Current treatments may lack effectiveness and produce dangerous side effects, hence a need for alternatives. Medicinal plants like <i>Millettia griffoniana</i> may possess anti-cancer properties. This study aimed to isolate and identify compounds from <i>M. griffoniana</i> to test for potential anti-cancer activity on pancreatic and colorectal cancer. Seeds and root bark of <i>M. griffoniana</i> underwent solvent extraction and separation using column chromatography. Isolated compounds were screened using AlamarBlue assays and cytotoxic compounds were identified with 2D NMR. Compounds were subject to cellular viability assays using AlamarBlue and xCELLigence analysis. The Caspase Glo®-3/7 kit measured caspase activity and Real-Time PCR analysis measured apoptosis-related gene expression. 7 compounds were isolated and screened. Compounds 5 and 7 were chosen; identified as durmillone and isojamaicin. Both showed varying concentration-dependent cytotoxic activity, in AlamarBlue and xCELLigence assays, for both cell lines. Caspases 3 and 7 were up-regulated and both compounds up-regulated <i>BAX</i> and down-regulated <i>BCL-2 a</i>nd <i>p53</i> in both cell lines. Durmillone and isojamaicin displayed cytotoxic activity on pancreatic and colorectal cancer. Apoptotic activity induced by the compounds was verified by the up-regulation of caspase activity and <i>BAX</i> and down-regulation of <i>BCL-2</i> and <i>p53</i>. Further studies need to be done towards understanding the mechanisms by which these compounds bring about their cytotoxic activity.</p></div>\",\"PeriodicalId\":7613,\"journal\":{\"name\":\"Advances in Traditional Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-01-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s13596-023-00733-y.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advances in Traditional Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13596-023-00733-y\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Traditional Medicine","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s13596-023-00733-y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
The anti-cancer potential of active compounds extracted from Millettia griffoniana on pancreatic and colorectal cancer cells
Pancreatic and colorectal cancer are two of the most lethal cancers; this stems from poor prognosis. Current treatments may lack effectiveness and produce dangerous side effects, hence a need for alternatives. Medicinal plants like Millettia griffoniana may possess anti-cancer properties. This study aimed to isolate and identify compounds from M. griffoniana to test for potential anti-cancer activity on pancreatic and colorectal cancer. Seeds and root bark of M. griffoniana underwent solvent extraction and separation using column chromatography. Isolated compounds were screened using AlamarBlue assays and cytotoxic compounds were identified with 2D NMR. Compounds were subject to cellular viability assays using AlamarBlue and xCELLigence analysis. The Caspase Glo®-3/7 kit measured caspase activity and Real-Time PCR analysis measured apoptosis-related gene expression. 7 compounds were isolated and screened. Compounds 5 and 7 were chosen; identified as durmillone and isojamaicin. Both showed varying concentration-dependent cytotoxic activity, in AlamarBlue and xCELLigence assays, for both cell lines. Caspases 3 and 7 were up-regulated and both compounds up-regulated BAX and down-regulated BCL-2 and p53 in both cell lines. Durmillone and isojamaicin displayed cytotoxic activity on pancreatic and colorectal cancer. Apoptotic activity induced by the compounds was verified by the up-regulation of caspase activity and BAX and down-regulation of BCL-2 and p53. Further studies need to be done towards understanding the mechanisms by which these compounds bring about their cytotoxic activity.
期刊介绍:
Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.