从 Millettia griffoniana 中提取的活性化合物对胰腺癌和结直肠癌细胞的抗癌潜力

IF 1.8 Q3 PHARMACOLOGY & PHARMACY Advances in Traditional Medicine Pub Date : 2024-01-08 DOI:10.1007/s13596-023-00733-y
Fatima Hoosen, Jordan Tonga Lembe, Derek Ndinteh, Tagatsing Fotsing Maurice, Yankep Emmanuel, Lesetja Raymond Motadi, Mpho Susan Choene
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引用次数: 0

摘要

胰腺癌和结肠直肠癌是致死率最高的两种癌症;这是因为它们的预后很差。目前的治疗方法可能缺乏有效性并产生危险的副作用,因此需要替代疗法。Millettia griffoniana 等药用植物可能具有抗癌特性。本研究旨在分离和鉴定 M. griffoniana 的化合物,以测试其对胰腺癌和结肠直肠癌的潜在抗癌活性。对 M. griffoniana 的种子和根皮进行溶剂萃取,并使用柱层析法进行分离。利用 AlamarBlue 分析法筛选分离出的化合物,并利用二维核磁共振鉴定细胞毒性化合物。使用 AlamarBlue 和 xCELLigence 分析法对化合物进行细胞活力检测。Caspase Glo®-3/7 试剂盒测定了 Caspase 活性,Real-Time PCR 分析测定了细胞凋亡相关基因的表达。分离并筛选出 7 种化合物。化合物 5 和 7 被选中,分别被鉴定为 durmillone 和 isojamaicin。在 AlamarBlue 和 xCELLigence 试验中,这两种化合物对两种细胞株都显示出不同浓度依赖性的细胞毒性活性。在两种细胞系中,Caspase 3 和 7 均上调,两种化合物均上调 BAX,下调 BCL-2 和 p53。Durmillone和isojamaicin对胰腺癌和结直肠癌具有细胞毒性。化合物诱导的凋亡活性通过 caspase 活性和 BAX 的上调以及 BCL-2 和 p53 的下调得到了验证。要了解这些化合物产生细胞毒性活性的机制,还需要做进一步的研究。
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The anti-cancer potential of active compounds extracted from Millettia griffoniana on pancreatic and colorectal cancer cells

Pancreatic and colorectal cancer are two of the most lethal cancers; this stems from poor prognosis. Current treatments may lack effectiveness and produce dangerous side effects, hence a need for alternatives. Medicinal plants like Millettia griffoniana may possess anti-cancer properties. This study aimed to isolate and identify compounds from M. griffoniana to test for potential anti-cancer activity on pancreatic and colorectal cancer. Seeds and root bark of M. griffoniana underwent solvent extraction and separation using column chromatography. Isolated compounds were screened using AlamarBlue assays and cytotoxic compounds were identified with 2D NMR. Compounds were subject to cellular viability assays using AlamarBlue and xCELLigence analysis. The Caspase Glo®-3/7 kit measured caspase activity and Real-Time PCR analysis measured apoptosis-related gene expression. 7 compounds were isolated and screened. Compounds 5 and 7 were chosen; identified as durmillone and isojamaicin. Both showed varying concentration-dependent cytotoxic activity, in AlamarBlue and xCELLigence assays, for both cell lines. Caspases 3 and 7 were up-regulated and both compounds up-regulated BAX and down-regulated BCL-2 and p53 in both cell lines. Durmillone and isojamaicin displayed cytotoxic activity on pancreatic and colorectal cancer. Apoptotic activity induced by the compounds was verified by the up-regulation of caspase activity and BAX and down-regulation of BCL-2 and p53. Further studies need to be done towards understanding the mechanisms by which these compounds bring about their cytotoxic activity.

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来源期刊
Advances in Traditional Medicine
Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-
CiteScore
4.30
自引率
0.00%
发文量
50
期刊介绍: Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
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