microRNA-9过表达对抑制黑色素瘤癌干细胞致瘤性的影响

IF 1.5 4区 医学 Q3 DERMATOLOGY Melanoma Research Pub Date : 2024-04-01 Epub Date: 2024-01-15 DOI:10.1097/CMR.0000000000000931
Sahranavardfard Parisa, Izadpanah Amirhossein, Yasavoli-Sharahi Hamed, Firouzi Javad, Azimi Masoumeh, Khosravani Pardis, Dorraj Mahshad, Keighobadi Faezeh, Ebrahimi Marzieh
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引用次数: 0

摘要

与原发肿瘤相比,大多数研究都报道了转移性黑色素瘤中 miR-9 的下调。他们指出,miR-9通过抑制SNAIL1的表达负向调节上皮细胞向间质转化(EMT),从而促进CDH1的表达。由于 EMT 过程与干细胞特征有关,研究 miR-9 对黑色素瘤干细胞的影响可能很有意义。在本研究中,我们考察了操纵 miR-9 对黑色素瘤细胞干性潜能的影响。我们的数据表明,miR-9在A375和NA8细胞中的过表达会显著抑制黑色素瘤细胞的增殖、自我更新、迁移和致瘤性能力,并伴随着BRAF、一些EMT因子和干性基因水平的变化。同样,miR-9 水平的降低会导致细胞增殖、集落和球体形成、细胞迁移能力和致瘤性的增加。总之,我们的研究结果明确了miR-9作为肿瘤抑制miRNA对黑色素瘤干细胞多方面的抑制作用,因此,它可能是抑制黑色素瘤生长和进展的潜在候选者。
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The effect of microRNA-9 overexpression on inhibition of melanoma cancer stem cells tumorigenicity.

Most of the studies have reported the downregulation of miR-9 in metastatic melanomas compared to primary tumors. They indicated that miR-9 negatively regulates the epithelial-to-mesenchymal transition (EMT) by inhibiting SNAIL1 expression and consequently promotes CDH1 expression. Since the process of EMT is associated to stem cell features, it could be interesting to study the effect of miR-9 on melanoma cancer stem cells. In the present study, we examined the effects of miR-9 manipulation on the stemness potential of melanoma cells. Our data demonstrated that the overexpression of miR-9 in A375 and NA8 cells significantly inhibits the ability of proliferation, self-renewal, migration, and tumorigenicity of melanoma cells which was concomitant with changes in the level of BRAF , some EMT factors, and stemness genes. Likewise, the reduction of miR-9 levels led to an increase in cell proliferation, colony and sphere formation, and the ability of cell migration and tumorigenicity. In conclusion, our results specified the role of miR-9 as a tumor suppressor miRNA to inhibit many aspects of melanoma stem cells, and therefore, it could be a potential candidate for the suppression of melanoma growth and progression.

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来源期刊
Melanoma Research
Melanoma Research 医学-皮肤病学
CiteScore
3.40
自引率
4.50%
发文量
139
审稿时长
6-12 weeks
期刊介绍: ​​​​​​Melanoma Research is a well established international forum for the dissemination of new findings relating to melanoma. The aim of the Journal is to promote the level of informational exchange between those engaged in the field. Melanoma Research aims to encourage an informed and balanced view of experimental and clinical research and extend and stimulate communication and exchange of knowledge between investigators with differing areas of expertise. This will foster the development of translational research. The reporting of new clinical results and the effect and toxicity of new therapeutic agents and immunotherapy will be given emphasis by rapid publication of Short Communications. ​Thus, Melanoma Research seeks to present a coherent and up-to-date account of all aspects of investigations pertinent to melanoma. Consequently the scope of the Journal is broad, embracing the entire range of studies from fundamental and applied research in such subject areas as genetics, molecular biology, biochemistry, cell biology, photobiology, pathology, immunology, and advances in clinical oncology influencing the prevention, diagnosis and treatment of melanoma.
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