Parita T. Suwan MD, Ga Ram Ahn MD, PhD, Roger Sumner MSc, Dilip Paithankar PhD, Ilya V. Yaroslavsky PhD, Gregory Altshuler PhD, Valeriya Arkhipova PhD, Dieter Manstein MD, PhD, Michael Wang-Evers PhD
{"title":"用于激光辅助给药的新型 40 µm 光斑尺寸 3050/3200 nm DFG 激光器与 CO2 激光器的对比。","authors":"Parita T. Suwan MD, Ga Ram Ahn MD, PhD, Roger Sumner MSc, Dilip Paithankar PhD, Ilya V. Yaroslavsky PhD, Gregory Altshuler PhD, Valeriya Arkhipova PhD, Dieter Manstein MD, PhD, Michael Wang-Evers PhD","doi":"10.1002/lsm.23755","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background and Objectives</h3>\n \n <p>The use of ablative fractional lasers to enhance the delivery of topical drugs through the skin is known as laser-assisted drug delivery. Here, we compare a novel 3050/3200 nm difference frequency generation (DFG) fiber laser (spot size: 40 µm) to a commercially used CO<sub>2</sub> laser (spot size: 120 µm). The objective is to determine whether differences in spot size and coagulation zone (CZ) thickness influence drug uptake.</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>Fractional ablation was performed on ex-vivo human abdominal skin with the DFG (5 mJ) and CO<sub>2</sub> (12 mJ) lasers to generate 680 µm deep lesions. To evaluate drug delivery, 30 kDa encapsulated fluorescent dye was topically applied to the skin and histologically analyzed at skin depths of 100, 140, 200, 400, and 600 µm. Additionally, transcutaneous permeation of encapsulated and 350 Da nonencapsulated dye was assessed using Franz Cells.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The DFG laser generated smaller channels (diameter: 56.5 µm) with thinner CZs (thickness: 22.4 µm) than the CO<sub>2</sub> laser (diameter: 75.9 µm, thickness: 66.8 µm). The DFG laser treated group exhibited significantly higher encapsulated dye total fluorescence intensities after 3 h compared to the CO<sub>2</sub> laser treated group across all skin depths (<i>p</i> < 0.001). Permeation of nonencapsulated dye was also higher in the DFG laser treated group vs the CO<sub>2</sub> laser treated group after 48 h (<i>p</i> < 0.0001), while encapsulated dye was not detected in any group.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The DFG laser treated skin exhibited significantly higher total fluorescence uptake compared to the CO<sub>2</sub> laser. Additionally, the smaller spot size and thinner CZ of the DFG laser could result in faster wound healing and reduced adverse effects while delivering similar or greater amount of topically applied drugs.</p>\n </section>\n </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"56 2","pages":"186-196"},"PeriodicalIF":2.2000,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lsm.23755","citationCount":"0","resultStr":"{\"title\":\"Novel 40 µm spot size 3050/3200 nm DFG laser versus CO2 laser for laser-assisted drug delivery\",\"authors\":\"Parita T. Suwan MD, Ga Ram Ahn MD, PhD, Roger Sumner MSc, Dilip Paithankar PhD, Ilya V. Yaroslavsky PhD, Gregory Altshuler PhD, Valeriya Arkhipova PhD, Dieter Manstein MD, PhD, Michael Wang-Evers PhD\",\"doi\":\"10.1002/lsm.23755\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background and Objectives</h3>\\n \\n <p>The use of ablative fractional lasers to enhance the delivery of topical drugs through the skin is known as laser-assisted drug delivery. Here, we compare a novel 3050/3200 nm difference frequency generation (DFG) fiber laser (spot size: 40 µm) to a commercially used CO<sub>2</sub> laser (spot size: 120 µm). The objective is to determine whether differences in spot size and coagulation zone (CZ) thickness influence drug uptake.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Materials and Methods</h3>\\n \\n <p>Fractional ablation was performed on ex-vivo human abdominal skin with the DFG (5 mJ) and CO<sub>2</sub> (12 mJ) lasers to generate 680 µm deep lesions. To evaluate drug delivery, 30 kDa encapsulated fluorescent dye was topically applied to the skin and histologically analyzed at skin depths of 100, 140, 200, 400, and 600 µm. Additionally, transcutaneous permeation of encapsulated and 350 Da nonencapsulated dye was assessed using Franz Cells.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The DFG laser generated smaller channels (diameter: 56.5 µm) with thinner CZs (thickness: 22.4 µm) than the CO<sub>2</sub> laser (diameter: 75.9 µm, thickness: 66.8 µm). The DFG laser treated group exhibited significantly higher encapsulated dye total fluorescence intensities after 3 h compared to the CO<sub>2</sub> laser treated group across all skin depths (<i>p</i> < 0.001). 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Novel 40 µm spot size 3050/3200 nm DFG laser versus CO2 laser for laser-assisted drug delivery
Background and Objectives
The use of ablative fractional lasers to enhance the delivery of topical drugs through the skin is known as laser-assisted drug delivery. Here, we compare a novel 3050/3200 nm difference frequency generation (DFG) fiber laser (spot size: 40 µm) to a commercially used CO2 laser (spot size: 120 µm). The objective is to determine whether differences in spot size and coagulation zone (CZ) thickness influence drug uptake.
Materials and Methods
Fractional ablation was performed on ex-vivo human abdominal skin with the DFG (5 mJ) and CO2 (12 mJ) lasers to generate 680 µm deep lesions. To evaluate drug delivery, 30 kDa encapsulated fluorescent dye was topically applied to the skin and histologically analyzed at skin depths of 100, 140, 200, 400, and 600 µm. Additionally, transcutaneous permeation of encapsulated and 350 Da nonencapsulated dye was assessed using Franz Cells.
Results
The DFG laser generated smaller channels (diameter: 56.5 µm) with thinner CZs (thickness: 22.4 µm) than the CO2 laser (diameter: 75.9 µm, thickness: 66.8 µm). The DFG laser treated group exhibited significantly higher encapsulated dye total fluorescence intensities after 3 h compared to the CO2 laser treated group across all skin depths (p < 0.001). Permeation of nonencapsulated dye was also higher in the DFG laser treated group vs the CO2 laser treated group after 48 h (p < 0.0001), while encapsulated dye was not detected in any group.
Conclusion
The DFG laser treated skin exhibited significantly higher total fluorescence uptake compared to the CO2 laser. Additionally, the smaller spot size and thinner CZ of the DFG laser could result in faster wound healing and reduced adverse effects while delivering similar or greater amount of topically applied drugs.
期刊介绍:
Lasers in Surgery and Medicine publishes the highest quality research and clinical manuscripts in areas relating to the use of lasers in medicine and biology. The journal publishes basic and clinical studies on the therapeutic and diagnostic use of lasers in all the surgical and medical specialties. Contributions regarding clinical trials, new therapeutic techniques or instrumentation, laser biophysics and bioengineering, photobiology and photochemistry, outcomes research, cost-effectiveness, and other aspects of biomedicine are welcome. Using a process of rigorous yet rapid review of submitted manuscripts, findings of high scientific and medical interest are published with a minimum delay.