细菌枯萎病影响土壤-根系连续体微生物群落的结构和组合

IF 6.2 2区 环境科学与生态学 Q1 GENETICS & HEREDITY Environmental Microbiome Pub Date : 2024-01-16 DOI:10.1186/s40793-024-00548-7
Jinchang Liang, Chengjian Wei, Xueru Song, Rui Wang, Heli Shi, Jun Tan, Dejie Cheng, Wenjing Wang, Xiaoqiang Wang
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引用次数: 0

摘要

有益的根相关微生物群在促进植物生长和抑制病原体威胁方面发挥着至关重要的作用,它们在抵御病原体方面的应用已引起越来越多的关注。然而,病原体入侵时微生物组的组装动态和防御机制在很大程度上仍然未知。在这项研究中,我们的目的是研究在细菌性植物病原体 Ralstonia solanacearum 的影响下,四个壁龛(大块土壤、根瘤层、根瘤表面和内层)中微生物群落的多样性和组装情况。我们的研究结果表明,与患病烟草植物相比,健康烟草植物在根相关壁龛中表现出更多样化的群落组成和更强大的共生网络。随机过程(扩散限制和漂移)而非确定性主导了群落的集结过程,在病株中观察到的漂移影响比在健康植株中更大。此外,在茄腐镰刀菌入侵期间,病株中镰刀菌属的数量显著增加,而镰刀菌属是已知的镰刀菌枯萎病的潜在病原体。此外,病株和健康植株的微生物组对病原体的反应策略也不尽相同。生病的微生物群组会招募有益的微生物类群,如链霉菌和芽孢杆菌,以抵御病原体,微生物类群的增加与病原体呈负相关。与此相反,健康植物的潜在防御策略在不同的生境中也各不相同,根瘤层中与生物膜形成相关的功能基因和内球层中与抗生素生物合成相关的功能基因显著富集。我们的研究揭示了健康烟草植株和患病烟草植株在土壤-根系连续过程中不同的群落组成和微生物群落的组装机制,为了解特定生态位的病原体入侵防御机制提供了新的视角。这些发现可能强调了利用不同功能的益生元提高植物抵御病原体能力的潜力。
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Bacterial wilt affects the structure and assembly of microbial communities along the soil-root continuum
Beneficial root-associated microbiomes play crucial roles in enhancing plant growth and suppressing pathogenic threats, and their application for defending against pathogens has garnered increasing attention. Nonetheless, the dynamics of microbiome assembly and defense mechanisms during pathogen invasion remain largely unknown. In this study, we aimed to investigate the diversity and assembly of microbial communities within four niches (bulk soils, rhizosphere, rhizoplane, and endosphere) under the influence of the bacterial plant pathogen Ralstonia solanacearum. Our results revealed that healthy tobacco plants exhibited more diverse community compositions and more robust co-occurrence networks in root-associated niches compared to diseased tobacco plants. Stochastic processes (dispersal limitation and drift), rather than determinism, dominated the assembly processes, with a higher impact of drift observed in diseased plants than in healthy ones. Furthermore, during the invasion of R. solanacearum, the abundance of Fusarium genera, a known potential pathogen of Fusarium wilt, significantly increased in diseased plants. Moreover, the response strategies of the microbiomes to pathogens in diseased and healthy plants diverged. Diseased microbiomes recruited beneficial microbial taxa, such as Streptomyces and Bacilli, to mount defenses against pathogens, with an increased presence of microbial taxa negatively correlated with the pathogen. Conversely, the potential defense strategies varied across niches in healthy plants, with significant enrichments of functional genes related to biofilm formation in the rhizoplane and antibiotic biosynthesis in the endosphere. Our study revealed the varied community composition and assembly mechanism of microbial communities between healthy and diseased tobacco plants along the soil-root continuum, providing new insights into niche-specific defense mechanisms against pathogen invasions. These findings may underscore the potential utilization of different functional prebiotics to enhance plants’ ability to fend off pathogens.
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来源期刊
Environmental Microbiome
Environmental Microbiome Immunology and Microbiology-Microbiology
CiteScore
7.40
自引率
2.50%
发文量
55
审稿时长
13 weeks
期刊介绍: Microorganisms, omnipresent across Earth's diverse environments, play a crucial role in adapting to external changes, influencing Earth's systems and cycles, and contributing significantly to agricultural practices. Through applied microbiology, they offer solutions to various everyday needs. Environmental Microbiome recognizes the universal presence and significance of microorganisms, inviting submissions that explore the diverse facets of environmental and applied microbiological research.
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