从尼沃鲁伯链霉菌（Streptomyces niveoruber）中分离出的五种新的链霉菌素抗生素类似物--Viridogrisein。

IF 1.5 4区医学 Q4 CHEMISTRY, MEDICINAL Chemical & pharmaceutical bulletin Pub Date : 2024-01-01 DOI:10.1248/cpb.c23-00776

Aya Yoshimura, Takumi Honda, Toshiyuki Wakimoto

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引用次数: 0

摘要

从尼沃鲁伯链霉菌（Streptomyces niveoruber）中分离出了五种新的病毒灵素 B-F，它们与病毒灵素（viridogrisein）和格里索维灵素（griseoviridin）同属于链霉菌素家族抗生素。通过液相色谱-串联质谱（LC-MS/MS）分析和先进的马菲法（Marfey's method）相结合的方法，阐明了viridogriseins B-F的结构，每种物质都具有不同的氨基酸组成。与其他链格列净家族抗生素一样，这些病毒唑类似物对金黄色葡萄球菌具有很强的抗菌活性。此外，每种病毒唑类似物与格列卫苷的等摩尔混合物对金黄色葡萄球菌生长的抑制作用比单独使用每种类似物更强。最后，对病毒性利血平生物合成基因簇中编码的 SgvY 进行体外功能分析后发现，SgvY 通过线性化作用对金黄色葡萄球菌的病毒性利血平进行解毒。考虑到viridogrisein对尼沃鲁伯氏菌没有自体毒性，SgvY很可能是尼沃鲁伯氏菌对viridogrisein的自我抵抗系统的一部分。

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Five New Analogs of Streptogramin Antibiotic Viridogrisein Isolated from Streptomyces niveoruber.

Five new viridogriseins B-F were isolated from Streptomyces niveoruber, along with viridogrisein and griseoviridin which belong to streptogramin family antibiotics. A combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis and the advanced Marfey's method elucidated the structures of viridogriseins B-F, each featuring distinct constituent amino acids. Consistent with other streptogramin family antibiotics, these viridogrisein analogs exhibited potent antibacterial activity against Staphylococcus aureus. Furthermore, equimolar mixtures of each viridogrisein analog and griseoviridin inhibited the growth of S. aureus more potently than each analog treatment alone. Finally, an in vitro functional analysis of SgvY, encoded in the viridogrisein biosynthetic gene cluster, revealed that SgvY detoxifies viridogrisein against S. aureus by linearization. Considering that viridogrisein is not autotoxic to S. niveoruber, SgvY likely contributes to the self-resistance system against viridogrisein in S. niveoruber.

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来源期刊

Chemical & pharmaceutical bulletin 医学-化学综合

CiteScore

3.20

自引率

5.90%

发文量

132

审稿时长

1.7 months

期刊介绍： The CPB covers various chemical topics in the pharmaceutical and health sciences fields dealing with biologically active compounds, natural products, and medicines, while BPB deals with a wide range of biological topics in the pharmaceutical and health sciences fields including scientific research from basic to clinical studies. For details of their respective scopes, please refer to the submission topic categories below. Topics: Organic chemistry In silico science Inorganic chemistry Pharmacognosy Health statistics Forensic science Biochemistry Pharmacology Pharmaceutical care and science Medicinal chemistry Analytical chemistry Physical pharmacy Natural product chemistry Toxicology Environmental science Molecular and cellular biology Biopharmacy and pharmacokinetics Pharmaceutical education Chemical biology Physical chemistry Pharmaceutical engineering Epidemiology Hygiene Regulatory science Immunology and microbiology Clinical pharmacy Miscellaneous.