Zuzana Cieslarova, Maria F. Mora*, Aaron C. Noell, Ceth W. Parker and Peter A. Willis,
{"title":"pH 值对通过亚临界水萃取从微生物中释放氨基酸的影响","authors":"Zuzana Cieslarova, Maria F. Mora*, Aaron C. Noell, Ceth W. Parker and Peter A. Willis, ","doi":"10.1021/acsearthspacechem.3c00277","DOIUrl":null,"url":null,"abstract":"<p >Distributions of amino acids, based on either enantiomeric excess or relative abundances of amino acid type, are key chemical measurements in the search for life on potential future astrobiology missions. This study investigates the impact of subcritical water extraction (SCWE) at different pH values on the measurement of these amino acid distributions when the starting material contains microorganisms. Cells of <i>Pseudoalteromonas haloplanktis</i> at pH 2, 6, and 12 underwent SCWE at 200 °C for 30 min, and the released amino acids were analyzed by capillary electrophoresis with laser-induced fluorescence detection. The overall extraction yield was improved under both acidic and alkaline conditions relative to neutral ones. The characteristic biotic distributions of amino acid types present in these samples were preserved under all conditions, although some degradation was observed as a relative increase in the amount of simpler amino acids like glycine. Contrarily, while biotic enantiomeric excesses remained preserved under acidic conditions, racemization was observed under alkaline conditions. This work shows the power of SCWE for converting polypeptide biopolymers into amino acid monomers, independent of the initial pH of the sample. However, it also highlights the need to understand the physiochemical properties of the sample to allow the mitigation of undesirable changes to the native distributions of amino acids.</p>","PeriodicalId":15,"journal":{"name":"ACS Earth and Space Chemistry","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of pH on the Release of Amino Acids from Microorganisms via Subcritical Water Extraction\",\"authors\":\"Zuzana Cieslarova, Maria F. Mora*, Aaron C. Noell, Ceth W. Parker and Peter A. Willis, \",\"doi\":\"10.1021/acsearthspacechem.3c00277\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Distributions of amino acids, based on either enantiomeric excess or relative abundances of amino acid type, are key chemical measurements in the search for life on potential future astrobiology missions. This study investigates the impact of subcritical water extraction (SCWE) at different pH values on the measurement of these amino acid distributions when the starting material contains microorganisms. Cells of <i>Pseudoalteromonas haloplanktis</i> at pH 2, 6, and 12 underwent SCWE at 200 °C for 30 min, and the released amino acids were analyzed by capillary electrophoresis with laser-induced fluorescence detection. The overall extraction yield was improved under both acidic and alkaline conditions relative to neutral ones. The characteristic biotic distributions of amino acid types present in these samples were preserved under all conditions, although some degradation was observed as a relative increase in the amount of simpler amino acids like glycine. Contrarily, while biotic enantiomeric excesses remained preserved under acidic conditions, racemization was observed under alkaline conditions. This work shows the power of SCWE for converting polypeptide biopolymers into amino acid monomers, independent of the initial pH of the sample. However, it also highlights the need to understand the physiochemical properties of the sample to allow the mitigation of undesirable changes to the native distributions of amino acids.</p>\",\"PeriodicalId\":15,\"journal\":{\"name\":\"ACS Earth and Space Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-01-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Earth and Space Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acsearthspacechem.3c00277\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Earth and Space Chemistry","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsearthspacechem.3c00277","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Effect of pH on the Release of Amino Acids from Microorganisms via Subcritical Water Extraction
Distributions of amino acids, based on either enantiomeric excess or relative abundances of amino acid type, are key chemical measurements in the search for life on potential future astrobiology missions. This study investigates the impact of subcritical water extraction (SCWE) at different pH values on the measurement of these amino acid distributions when the starting material contains microorganisms. Cells of Pseudoalteromonas haloplanktis at pH 2, 6, and 12 underwent SCWE at 200 °C for 30 min, and the released amino acids were analyzed by capillary electrophoresis with laser-induced fluorescence detection. The overall extraction yield was improved under both acidic and alkaline conditions relative to neutral ones. The characteristic biotic distributions of amino acid types present in these samples were preserved under all conditions, although some degradation was observed as a relative increase in the amount of simpler amino acids like glycine. Contrarily, while biotic enantiomeric excesses remained preserved under acidic conditions, racemization was observed under alkaline conditions. This work shows the power of SCWE for converting polypeptide biopolymers into amino acid monomers, independent of the initial pH of the sample. However, it also highlights the need to understand the physiochemical properties of the sample to allow the mitigation of undesirable changes to the native distributions of amino acids.
期刊介绍:
The scope of ACS Earth and Space Chemistry includes the application of analytical, experimental and theoretical chemistry to investigate research questions relevant to the Earth and Space. The journal encompasses the highly interdisciplinary nature of research in this area, while emphasizing chemistry and chemical research tools as the unifying theme. The journal publishes broadly in the domains of high- and low-temperature geochemistry, atmospheric chemistry, marine chemistry, planetary chemistry, astrochemistry, and analytical geochemistry. ACS Earth and Space Chemistry publishes Articles, Letters, Reviews, and Features to provide flexible formats to readily communicate all aspects of research in these fields.