抗氧化剂丝胶可避免氧化应激引发的卵母细胞-卵泡细胞通讯中断。

IF 3.6 2区 医学 Q2 DEVELOPMENTAL BIOLOGY Molecular human reproduction Pub Date : 2024-02-01 DOI:10.1093/molehr/gaae001
Hafiza Khatun, Ken-Ichi Yamanaka, Satoshi Sugimura
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引用次数: 0

摘要

抗氧化剂是一种自由基清除剂,可通过抑制氧化应激提高卵母细胞质量并改善女性生育能力。然而,相关机制仍不清楚。本研究旨在探讨在牛模型中,使用抗氧化剂丝胶是否能减少氧化应激,从而扩大精原细胞(CC)与卵母细胞的交流并获得卵母细胞的发育。我们发现,在丝胶存在下成熟的积层细胞-卵母细胞复合体(COCs)的卵母细胞减数分裂成熟率显著提高(P<0.05)。
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Antioxidant sericin averts the disruption of oocyte-follicular cell communication triggered by oxidative stress.

Antioxidants are free radical scavengers that increase oocyte quality and improve female fertility by suppressing oxidative stress. However, the related mechanisms remain unclear. The present study was designed to examine whether a reduction of oxidative stress from using the antioxidant sericin led to expanded cumulus cell (CC)-oocyte communication and oocyte developmental acquisition in a bovine model. We found that cumulus-oocyte complexes (COCs) matured in the presence of sericin showed a significantly increased oocyte meiotic maturation rate (P < 0.01) and accelerated subsequent blastocyst formation, as more blastocysts were found at the hatched stage (P < 0.05) compared to that in the control group. In contrast to the control group, sericin suppressed H2O2 levels in COCs, resulting in a markedly enhanced CC-oocyte gap junction communication index and number of transzonal projections, which were preserved until 18 h of oocyte maturation. These findings indicate that sericin reduces disruption of oocyte-follicular cell communication induced by oxidative stress. Sericin consistently increased intra-oocyte glutathione (GSH) levels and reduced oocyte H2O2 levels (P < 0.05), both of which were ablated when GSH synthesis was inhibited by buthionine sulfoximide (an inhibitor of GSH synthesis). Furthermore, the inhibition of GSH synthesis counteracted the positive effects of sericin on subsequent embryo developmental competence (P < 0.01). Intra-oocyte GSH levels were positively associated with blastocyst development and quality. These outcomes demonstrate new perspectives for the improvement of oocyte quality in assisted reproductive technology and may contribute to developing treatment strategies for infertility and cancer.

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来源期刊
Molecular human reproduction
Molecular human reproduction 生物-发育生物学
CiteScore
8.30
自引率
0.00%
发文量
37
审稿时长
6-12 weeks
期刊介绍: MHR publishes original research reports, commentaries and reviews on topics in the basic science of reproduction, including: reproductive tract physiology and pathology; gonad function and gametogenesis; fertilization; embryo development; implantation; and pregnancy and parturition. Irrespective of the study subject, research papers should have a mechanistic aspect.
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