印度北部半山工业中心地下水微生物质量调查

Ajay K. Singh, S. K. Bhardwaj, Rajeev Kumar Aggarwal, Sunita Devi, Amit Guleri
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摘要

索兰地区是印度北部喜马偕尔邦工业发展迅速的地区,该地区频繁爆发影响人类健康的水媒疾病。为查明原因,对该地区的饮用地下水源进行了微生物评估。为此,进行了耐热大肠菌群研究和基因组分型。在 2018-19 年的季风和季风后季节,在选定的五个水源地采用多管发酵技术检测耐热大肠菌群。耐热大肠菌群的最可能数从每 100 毫升水 2-34 个不等,表明水源受到污染,季风月份的密度明显更高(p< 0.05)。在伊红亚甲蓝平板上,从所有水源中获得了四种不同的大肠菌群形态。其中,唯一一种被命名为纳拉格尔水菌株(NGW)的菌株从所有水源中都得到了回收,表明其普遍存在。根据生化和形态特征,该菌株被认定为大肠埃希氏菌。相反,通过分子鉴定(16S rRNA 核糖体分型),确定其为植物拉乌尔特氏菌(GenBank 编号:MK318824)。该菌株被存放在马哈拉施特拉邦(印度)浦那国家微生物资源中心的国家菌种保藏中心,保藏编号为 MCC 4064,以备将来在公共研究活动中使用。微生物学评估表明,饮用水源受到污染,可能会导致腹泻疾病。16S rRNA 核糖分型技术有助于正确识别微生物。
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An Investigation into the Microbiological Quality of Groundwater in an Industrial Hub in Mid-Hills of Northern India
Frequent water-borne disease outbreaks affecting human health have been reported in district Solan, which is a rapidly industrialing region of Himachal Pradesh, a northern state of India. For ascertaining the cause, microbiological assessment of drinking groundwater sources of the region was undertaken. For this, thermotolerant coliform study and genome typing were undertaken. Multiple tube fermentation technique was employed in selected five sources to detect thermotolerant coliforms during the seasons of monsoon and post-monsoon in the years 2018-19. Thermotolerant coliforms varied in Most Probable Number from 2-34 per 100 ml water indicating contaminated water sources, densities being significantly more in monsoon months (p< 0.05). Four different coliform morphotypes were obtained from all the sources on Eosin Methylene Blue plates. Of these, the only strain, designated as Nalagarh Water Strain (NGW), was recovered from all sources, suggesting its prevalence. Based upon biochemical and morphological attributes, the strain was recognized as Escherichia coli. Conversely, molecular characterization (16S rRNA ribotyping) identified it as Raoultella planticola (GenBank accession No. MK318824). The strain was deposited in a National Culture Collection Centre, National Centre for Microbial Resources, Pune, Maharashtra (India) with deposition accession number MCC 4064 for future utility in public research activities. Microbiological assessment evinced contaminated drinking water sources, probably causing diarrhoeal diseases. 16S rRNA ribotyping assisted in correct identification of the microorganism.
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