用于癌症治疗的 CRISPR/Cas 基因编辑和传递系统

Yingjie Li, Shiyao Zhou, Qinjie Wu, Changyang Gong
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摘要

CRISPR/Cas 系统因其简便、高效等优点而脱颖而出,成为生物医学领域(包括癌症治疗)极具吸引力的基因编辑工具。CRISPR/Cas 系统通过对肿瘤细胞或免疫细胞进行操作和工程改造,为癌症治疗带来了希望。然而,如何克服重重生理障碍,将CRISPR元件高效、准确地输送到靶细胞,一直是人们关注的问题。在这篇综述中,我们介绍了CRISPR/Cas系统的机制,总结了目前通过物理方法、病毒载体和非病毒载体传递CRISPR/Cas系统的策略,并介绍了目前CRISPR/Cas系统在癌症临床治疗中的应用。此外,我们还讨论了 CRISPR/Cas 系统递送方法的相关前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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CRISPR/Cas gene editing and delivery systems for cancer therapy
CRISPR/Cas systems stand out because of simplicity, efficiency, and other superiorities, thus becoming attractive and brilliant gene-editing tools in biomedical field including cancer therapy. CRISPR/Cas systems bring promises for cancer therapy through manipulating and engineering on tumor cells or immune cells. However, there have been concerns about how to overcome the numerous physiological barriers and deliver CRISPR components to target cells efficiently and accurately. In this review, we introduced the mechanisms of CRISPR/Cas systems, summarized the current delivery strategies of CRISPR/Cas systems by physical methods, viral vectors, and nonviral vectors, and presented the current application of CRISPR/Cas systems in cancer clinical treatment. Furthermore, we discussed prospects related to delivery approaches of CRISPR/Cas systems.
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