A. D. Lemyakin, K. Sabetova, A. A. Chaitsky, P. O. Shchegolev, L. S. Badanina
{"title":"科斯特罗马地区科斯特罗马和黑白品种奶牛甲状腺球蛋白基因多态性研究","authors":"A. D. Lemyakin, K. Sabetova, A. A. Chaitsky, P. O. Shchegolev, L. S. Badanina","doi":"10.32634/0869-8155-2024-378-1-52-59","DOIUrl":null,"url":null,"abstract":"Relevance. The genotype of the animal plays a significant role in realizing its productive potential, along with other factors affecting dairy productivity. Marker-oriented breeding (MAS) of cattle, which utilizes genetic markers associated with economically valuable traits, has gained popularity in the last two decades. One such marker is the thyroglobulin (TG) gene, which is involved in the production of a thyroid prohormone.Methods.The research was conducted on cows of Kostroma and black-and-white breeds at breeding farms in the Kostroma region. Genetic material was obtained from the tail vein of the cows using individual vacuum systems with an anticoagulant. The polymorphism of the TG gene (rs135751032) was determined using RT-PCR and detection by hybridization-fluorescence method. The results were processed using biometric analysis and calculation of population-genetic indicators.Results. In the populations of cows of the Kostroma and black-and-white breeds of the Kostroma region, the predominance of carriers of the CC genotype and the C allele of the thyroglobulin gene was established. At the same time, among cows of these breeds, there is a tendency to higher milk productivity in heterozygous animals. The nature of the associative relationship between TG genotypes and milk productivity indicators differed significantly for each individual herd. This indicates the need to study TG in combination with other DNA markers of milk productivity and metabolism. Statistically significant differences between cows of different TG genotypes were found only in full-aged animals, which perhaps indicates the prospects of using TG to determine the genetic potential of productive longevity of cows.","PeriodicalId":7465,"journal":{"name":"Agrarian science","volume":"88 22","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Research of thyroglobulin gene polymorphism in cows of Kostroma and black-and-white breeds of Kostroma region\",\"authors\":\"A. D. Lemyakin, K. Sabetova, A. A. Chaitsky, P. O. Shchegolev, L. S. Badanina\",\"doi\":\"10.32634/0869-8155-2024-378-1-52-59\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Relevance. The genotype of the animal plays a significant role in realizing its productive potential, along with other factors affecting dairy productivity. Marker-oriented breeding (MAS) of cattle, which utilizes genetic markers associated with economically valuable traits, has gained popularity in the last two decades. One such marker is the thyroglobulin (TG) gene, which is involved in the production of a thyroid prohormone.Methods.The research was conducted on cows of Kostroma and black-and-white breeds at breeding farms in the Kostroma region. Genetic material was obtained from the tail vein of the cows using individual vacuum systems with an anticoagulant. The polymorphism of the TG gene (rs135751032) was determined using RT-PCR and detection by hybridization-fluorescence method. The results were processed using biometric analysis and calculation of population-genetic indicators.Results. In the populations of cows of the Kostroma and black-and-white breeds of the Kostroma region, the predominance of carriers of the CC genotype and the C allele of the thyroglobulin gene was established. At the same time, among cows of these breeds, there is a tendency to higher milk productivity in heterozygous animals. The nature of the associative relationship between TG genotypes and milk productivity indicators differed significantly for each individual herd. This indicates the need to study TG in combination with other DNA markers of milk productivity and metabolism. Statistically significant differences between cows of different TG genotypes were found only in full-aged animals, which perhaps indicates the prospects of using TG to determine the genetic potential of productive longevity of cows.\",\"PeriodicalId\":7465,\"journal\":{\"name\":\"Agrarian science\",\"volume\":\"88 22\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-01-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agrarian science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32634/0869-8155-2024-378-1-52-59\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agrarian science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32634/0869-8155-2024-378-1-52-59","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
相关性。动物的基因型与影响奶牛生产率的其他因素一样,在发挥其生产潜力方面起着重要作用。以标记为导向的牛育种(MAS)利用与具有经济价值的性状相关的遗传标记,在过去二十年中得到了普及。其中一个标记是甲状腺球蛋白(TG)基因,该基因参与甲状腺原激素的生产。使用带有抗凝剂的独立真空系统从奶牛尾静脉获取遗传物质。采用 RT-PCR 和杂交荧光法检测 TG 基因(rs135751032)的多态性。结果采用生物计量分析和种群遗传指标计算方法进行处理。在科斯特罗马地区的科斯特罗马和黑白品种奶牛群体中,甲状腺球蛋白基因的 CC 基因型和 C 等位基因携带者占多数。同时,在这些品种的奶牛中,杂合子动物的产奶量呈上升趋势。在每个牛群中,甲状腺球蛋白基因型与产奶量指标之间的关联关系的性质差异很大。这表明有必要将 TG 与其他有关牛奶生产率和新陈代谢的 DNA 标记结合起来研究。不同 TG 基因型的奶牛之间仅在全龄动物中发现了统计学意义上的显著差异,这或许表明了利用 TG 确定奶牛生产寿命遗传潜力的前景。
Research of thyroglobulin gene polymorphism in cows of Kostroma and black-and-white breeds of Kostroma region
Relevance. The genotype of the animal plays a significant role in realizing its productive potential, along with other factors affecting dairy productivity. Marker-oriented breeding (MAS) of cattle, which utilizes genetic markers associated with economically valuable traits, has gained popularity in the last two decades. One such marker is the thyroglobulin (TG) gene, which is involved in the production of a thyroid prohormone.Methods.The research was conducted on cows of Kostroma and black-and-white breeds at breeding farms in the Kostroma region. Genetic material was obtained from the tail vein of the cows using individual vacuum systems with an anticoagulant. The polymorphism of the TG gene (rs135751032) was determined using RT-PCR and detection by hybridization-fluorescence method. The results were processed using biometric analysis and calculation of population-genetic indicators.Results. In the populations of cows of the Kostroma and black-and-white breeds of the Kostroma region, the predominance of carriers of the CC genotype and the C allele of the thyroglobulin gene was established. At the same time, among cows of these breeds, there is a tendency to higher milk productivity in heterozygous animals. The nature of the associative relationship between TG genotypes and milk productivity indicators differed significantly for each individual herd. This indicates the need to study TG in combination with other DNA markers of milk productivity and metabolism. Statistically significant differences between cows of different TG genotypes were found only in full-aged animals, which perhaps indicates the prospects of using TG to determine the genetic potential of productive longevity of cows.