α-生育酚对在卵磷脂和三聚氰胺蛋黄扩展剂中扩展的塔帕卡尔公牛精液冷冻质量参数的影响

Pub Date : 2024-01-18 DOI:10.12681/jhvms.31491

A. Channo, A. Kaka, AA Memon, MC Malhi, M. Bakhsh, Q. Kalwar, DH Kalhoro, A. Sethar, RA Jariko

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引用次数: 0

摘要

本实验使用了 4 头 4-5 岁的健康 Tharparkar 公牛。共收集了 52 次（n=13）射精，对运动能力、形态和存活率≥70% 的精液样本进行了处理，并将其扩展到 BIOXcellTM 和补充或不补充 α-生育酚（维生素 E）0.02 Mm/ml 的三羟基蛋黄扩展剂中。与 Tris+ α-生育酚（维生素 E）（74.69±0.94，86.13±0.79，82.13±1.20，81.40±0.77）相比，BIOXcellTM + α-生育酚（维生素 E）（79.09±0.87，86.13±0.79，82.13±1.20，81.40±0.77）在解冻前对活力、形态、存活率和膜完整性的评估显示质量参数有所改善。69±0.94、82.81±1.00、77.63±1.26、77.31±1.01）、BIOXcellTM（73.55±1.20、80.81±0.96、76.02±1.28、75.31±1.20）和 Tris（71.72±1.28、78.90±1.01、74.04±1.31、73.18±1.29）相比，质量指标分别有所改善。总之，补充α-生育酚（维生素 E）可改善在 BIOXcellTM 和三羟甲基氨基甲烷蛋黄扩展剂中扩展的 Tharparkar 公牛精液的解冻前质量指标，即活力、形态、存活率和膜完整性。

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Effects of α-Tocopherol on chilled quality parameters of Tharparkar bull semen extended in Lecithin and Tris based egg yolk extender

In this experiment, four healthy Tharparkar Cattle Bulls, 4-5 years aged were used. Total 52 (n=13) ejaculates were collected, and the semen samples having motility, morphology and viability ≥70% were processed and extended into BIOXcellTM and Tris Based Egg Yolk extender supplemented with and without α-tocopherol (Vitamin E) 0.02 Mm/ml. Pre-thawed assessment of motility, morphology, viability and membrane integrity with BIOXcellTM + α-tocopherol (Vitamin E) (79.09±0.87, 86.13±0.79, 82.13±1.20, 81.40±0.77) showed improved quality parameters as compared to Tris+ α-tocopherol (Vitamin E) (74.69±0.94, 82.81±1.00, 77.63±1.26, 77.31±1.01), BIOXcellTM (73.55±1.20, 80.81±0.96, 76.02±1.28, 75.31±1.20) and Tris (71.72±1.28, 78.90±1.01, 74.04±1.31, 73.18±1.29) respectively. In conclusion supplementation of α-tocopherol (Vitamin E) improves the Pre thawed quality parameters i.e. Motility, Morphology, Viability and Membrane Integrity of Tharparkar Bull semen extended in BIOXcellTM and Tris Based Egg Yolk extender.

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