[水牛的轮状病毒肠炎]。

Veterinarno-meditsinski nauki Pub Date : 1987-01-01
L Muniiappa, G K Georgiev, Kh E Kharalambiev
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引用次数: 0

摘要

免疫扩散法用于调查私人和公共农场的水牛(6个月以下的水牛犊牛和水牛小母牛)共492份血清样本。在73.4%的病例中,轮状病毒血清学试剂呈阳性。通过阻断酶联免疫吸附试验对相同样本进行研究,发现86.2%的阳性病例存在。结果表明,这类ELISA技术是一种更精确的方法来证明水牛群体特异性轮状病毒抗体。所获得的结果说明了在这个国家的水牛种群中广泛发生的轮状病毒感染。轮状病毒在6个月大的小牛中传播尤为严重。通过免疫扩散和酶联免疫吸附试验对46例腹泻水牛犊牛粪便样本进行了研究,发现其中多达7例存在轮状病毒。这无疑证明了这些制剂在水牛肠炎病因学中的作用。
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[Rotavirus enteritis in buffaloes].

Immunodiffusion was used to investigate a total of 492 serum samples of buffaloes--buffalo calves up to the age of 6 months and buffalo heifers--on both private and public farms. Positive serological reagents for rotaviruses were established in 73.4 per cent of the cases. The study of the same samples via the blocking ELISA test revealed the presence of 86.2 per cent positive cases. It was demonstrated that this type of the ELISA technique was a more precise method for the demonstration of group specific rotavirus antibodies in buffaloes. The results obtained spoke of the widely occurring rotavirus infections among the buffalo populations in this country. Particularly intense was the circulation of rotaviruses in buffalo calves aged up to six months. The study of 46 fecal samples from buffalo calves with diarrhea by means of the immunodiffusion and ELISA tests revealed that in as many as seven of them there were rotaviruses. This unquestionably demonstrated the participation of these agents in the etiology of enteritis in buffaloes.

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