Y. Verma, Y. Verma, M. Swamy, A. Nayak, A. Dubey, M. Jatav, Amishi, A. Khare
{"title":"贾巴尔布尔地区反刍动物中副结核分枝杆菌亚种的分子和血清学检测","authors":"Y. Verma, Y. Verma, M. Swamy, A. Nayak, A. Dubey, M. Jatav, Amishi, A. Khare","doi":"10.18805/ijar.b-5149","DOIUrl":null,"url":null,"abstract":"Background: Paratuberculosis is a chronic infectious disease of wild and domestic ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). The present study aimed to conduct the molecular and serological detection of MAP in ruminants of Jabalpur region. Methods: A total of 261 faecal, 124 blood and 51 tissue samples were collected from goats, buffaloes and cattle of the region irrespective of their age, sex and breed. ZN staining of faecal and tissue samples was performed as a preliminary screening test followed by anti-MAP antibodies detection in serum samples using indirect ELISA and IS900 PCR analysis of ZN positive faecal and tissue samples. Result: A pattern of acid-fast bacilli shedding in faecal samples of ruminants was obtained based on ZN staining with a majority of +1 shedders. In ELISA, 33.87% of serum samples were found positive for anti-MAP antibodies whereas, 22.50% ZN positive faecal samples and 100% ZN positive tissue samples were found positive in IS900 PCR. Therefore, a conclusion of moderately prevalent MAP infection in ruminants of the Jabalpur region can be drawn.\n","PeriodicalId":13410,"journal":{"name":"Indian Journal of Animal Research","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular and Serological Detection of Mycobacterium avium Subspecies Paratuberculosis in Ruminants of Jabalpur Region\",\"authors\":\"Y. Verma, Y. Verma, M. Swamy, A. Nayak, A. Dubey, M. Jatav, Amishi, A. Khare\",\"doi\":\"10.18805/ijar.b-5149\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Paratuberculosis is a chronic infectious disease of wild and domestic ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). The present study aimed to conduct the molecular and serological detection of MAP in ruminants of Jabalpur region. Methods: A total of 261 faecal, 124 blood and 51 tissue samples were collected from goats, buffaloes and cattle of the region irrespective of their age, sex and breed. ZN staining of faecal and tissue samples was performed as a preliminary screening test followed by anti-MAP antibodies detection in serum samples using indirect ELISA and IS900 PCR analysis of ZN positive faecal and tissue samples. Result: A pattern of acid-fast bacilli shedding in faecal samples of ruminants was obtained based on ZN staining with a majority of +1 shedders. In ELISA, 33.87% of serum samples were found positive for anti-MAP antibodies whereas, 22.50% ZN positive faecal samples and 100% ZN positive tissue samples were found positive in IS900 PCR. Therefore, a conclusion of moderately prevalent MAP infection in ruminants of the Jabalpur region can be drawn.\\n\",\"PeriodicalId\":13410,\"journal\":{\"name\":\"Indian Journal of Animal Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2024-01-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Animal Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.18805/ijar.b-5149\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Animal Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.18805/ijar.b-5149","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Molecular and Serological Detection of Mycobacterium avium Subspecies Paratuberculosis in Ruminants of Jabalpur Region
Background: Paratuberculosis is a chronic infectious disease of wild and domestic ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). The present study aimed to conduct the molecular and serological detection of MAP in ruminants of Jabalpur region. Methods: A total of 261 faecal, 124 blood and 51 tissue samples were collected from goats, buffaloes and cattle of the region irrespective of their age, sex and breed. ZN staining of faecal and tissue samples was performed as a preliminary screening test followed by anti-MAP antibodies detection in serum samples using indirect ELISA and IS900 PCR analysis of ZN positive faecal and tissue samples. Result: A pattern of acid-fast bacilli shedding in faecal samples of ruminants was obtained based on ZN staining with a majority of +1 shedders. In ELISA, 33.87% of serum samples were found positive for anti-MAP antibodies whereas, 22.50% ZN positive faecal samples and 100% ZN positive tissue samples were found positive in IS900 PCR. Therefore, a conclusion of moderately prevalent MAP infection in ruminants of the Jabalpur region can be drawn.
期刊介绍：
The IJAR, the flagship print journal of ARCC, it is a monthly journal published without any break since 1966. The overall aim of the journal is to promote the professional development of its readers, researchers and scientists around the world. Indian Journal of Animal Research is peer-reviewed journal and has gained recognition for its high standard in the academic world. It anatomy, nutrition, production, management, veterinary, fisheries, zoology etc. The objective of the journal is to provide a forum to the scientific community to publish their research findings and also to open new vistas for further research. The journal is being covered under international indexing and abstracting services.
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