胸腺醌对大鼠肝脏缺血再灌注模型中瞬时受体电位美拉辛(TRPM)通道的影响

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI:10.22038/IJBMS.2023.71990.15647
Kerem Caglar, Recep Dokuyucu, Gokhan Agturk, Cemil Tumer, Okan Tutuk, Hatice Dogan Gocmen, Hasan Gokce, Zeynel Abidin Tas, Oguzhan Ozcan, Bulent Gogebakan
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引用次数: 0

摘要

研究目的我们旨在研究瞬时受体电位美司他丁(TRPM)基因表达水平,以及胸腺醌(Tmq)在肝脏I/R大鼠模型中的抗氧化和组织病理学效应:50 只 Wistar 大鼠分为 5 组。第 1 组:对照组;第 2 组:Sham 组;第 3 组:肝脏 I/R(45 分钟/45 分钟)组;第 4 组:Tmq(50 毫克/千克)组;第 5 组:Tmq+I/R(I/R 前十天,Tmq 剂量为 50 毫克/千克)组。缺血组在门静脉和肝动脉处用无创伤血管钳进行肝脏 I/R(45min/45min)模型。研究结束时采集的肝组织和血液样本将进行组织病理学和生化分析。此外,还测定了肝组织中 TRPM 基因的表达水平。结果显示,在组织病理学评估中,与 I/R 组相比,Tmq 组和 Tmq+I/R 组的细胞肿胀、充血、PNL 和细胞凋亡参数均有统计学下降:观察发现,与假体组、Tmq 组和 Tmq+I/R 组相比,I/R 组的生化指标、AST、ALT、GGT、LDH、肌酐和尿素水平明显升高。研究发现,与 I/R 组相比,Tmq+I/R 组的 TRPM2、6、7、8 基因表达明显下降:我们的研究表明,胸腺醌可通过降低TRPM2,6,7,8基因的表达来抑制Ca+2进入细胞。根据我们的研究结果,我们认为应用胸腺醌治疗因I/R损伤引起的肝脏疾病可能在缺血和细胞凋亡方面具有重要意义,也可用于治疗肝脏相关疾病。
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Effect of thymoquinone on transient receptor potential melastatin (TRPM) channels in rats with liver ischemia reperfusion model in rats.

Objectives: We aimed to investigate the levels of transient receptor potential melastatin (TRPM) gene expression, and the antioxidant and histopathologic effect of thymoquinone (Tmq) in the hepatic I/R rat model.

Materials and methods: Fifty Wistar rats were divided into 5 groups. Group 1: Control; Group 2: Sham; Group 3: Hepatic I/R (45 min/45 min); Group 4: Tmq (50 mg/kg); Group 5: Tmq+I/R (ten days before I/R at the dose of 50 mg/kg of Tmq). The hepatic I/R (45min/45min) model was performed at the portal vein and the hepatic artery with atraumatic vascular clamp in the ischemia groups. The liver tissues and blood samples that were taken at the end of the study were evaluated for histopathologic and biochemical analysis. Besides TRPM gene expression levels were determined in liver tissues. It was seen that cellular swelling, congestion, PNL, and apoptosis parameters statistically decreased in Tmq and Tmq+I/R groups in comparison with the I/R group in histopathological evaluation.

Results: It was observed that biochemical parameters, AST, ALT, GGT, LDH, creatinine, and urea levels significantly increased in the I/R group as compared with, sham, Tmq, and Tmq+I/R groups. It was found that TRPM2,6,7,8 gene expression decreased significantly in Tmq+I/R groups as compared to the I/R group.

Conclusion: We showed that thymoquinone can inhibit the entry of Ca+2 into the cell by decreasing TRPM2,6,7,8 gene expression. Based on our findings, we think that Tmq application in the treatment of liver diseases due to I/R damage may be important in terms of both ischemia and apoptosis and can also be used in the treatment of liver-related diseases.

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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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