{"title":"多巴胺和褪黑素处理对日本鳗鲡人工诱导性成熟相关基因表达的影响","authors":"Jun-Hwan Byun, Ji-Yeon Hyeon, Sachithra Amarin Hettiarachchi, Shingo Udagawa, Angka Mahardini, Jong-Myoung Kim, Sung-Pyo Hur, Akihiro Takemura","doi":"10.1002/jez.2788","DOIUrl":null,"url":null,"abstract":"<p>Japanese eel (<i>Anguilla japonica</i>) is a commercially important fish species in Asia. Understanding factors like photoperiod, temperature, and lunar cycles is crucial for successful aquaculture and managing its reproduction. Melatonin and dopamine (DA) are essential for regulating reproduction in vertebrates, including fish. This study investigated the effects of melatonin and DA on the reproductive system of mature male Japanese eels to better understand reproductive regulation in fish. To clarify the effects of these hormones on sexual maturation in eels, a critical stage in the reproductive process, sexual maturation was induced by injecting human chorionic gonadotropin, which stimulates the production of sex hormones. To check the effect of melatonin and DA on sexual maturation, DA, melatonin, and DA + domperidone were intraperitoneally injected into fish from each group (six per treatment) at a dose of 1 mg/kg body weight. The fish were then examined using quantitative RT-PCR by comparing the messenger RNA level of reproduction-related genes (gonadotropin releasing hormone 1; <i>gnrh1</i>, gonadotropin releasing hormone 2; <i>gnrh2</i>, follicle stimulating hormone; <i>fshβ</i>, luteinizing hormone; <i>lhβ</i> and DA receptor 2b; <i>d2b</i>), involved in the gonadotropic axis in eels, to those that received a control injection. The results indicate significant differences in the expression levels of <i>gnrh1</i>, <i>gnrh2</i> and <i>d2b</i> in the brain and <i>d2b</i>, <i>fshβ</i>, <i>lhβ</i> in the pituitary at different stages of sexual maturation. Melatonin appears to enhance the production of sex gonadotropins, whereas DA inhibits them. These findings suggest an interaction between melatonin and DA in regulating reproduction in Japanese eels.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of dopamine and melatonin treatment on the expression of the genes associated with artificially induced sexual maturation in Japanese eel, Anguilla japonica\",\"authors\":\"Jun-Hwan Byun, Ji-Yeon Hyeon, Sachithra Amarin Hettiarachchi, Shingo Udagawa, Angka Mahardini, Jong-Myoung Kim, Sung-Pyo Hur, Akihiro Takemura\",\"doi\":\"10.1002/jez.2788\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Japanese eel (<i>Anguilla japonica</i>) is a commercially important fish species in Asia. 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The fish were then examined using quantitative RT-PCR by comparing the messenger RNA level of reproduction-related genes (gonadotropin releasing hormone 1; <i>gnrh1</i>, gonadotropin releasing hormone 2; <i>gnrh2</i>, follicle stimulating hormone; <i>fshβ</i>, luteinizing hormone; <i>lhβ</i> and DA receptor 2b; <i>d2b</i>), involved in the gonadotropic axis in eels, to those that received a control injection. The results indicate significant differences in the expression levels of <i>gnrh1</i>, <i>gnrh2</i> and <i>d2b</i> in the brain and <i>d2b</i>, <i>fshβ</i>, <i>lhβ</i> in the pituitary at different stages of sexual maturation. Melatonin appears to enhance the production of sex gonadotropins, whereas DA inhibits them. These findings suggest an interaction between melatonin and DA in regulating reproduction in Japanese eels.</p>\",\"PeriodicalId\":15711,\"journal\":{\"name\":\"Journal of experimental zoology. 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引用次数: 0
摘要
日本鳗鱼(Anguilla japonica)是亚洲的一种重要商业鱼类。了解光周期、温度和月经周期等因素对成功养殖和管理其繁殖至关重要。褪黑激素和多巴胺(DA)对调节脊椎动物(包括鱼类)的繁殖至关重要。本研究调查了褪黑激素和多巴胺对成熟雄性日本鳗鱼生殖系统的影响,以更好地了解鱼类的生殖调节。性成熟是鳗鱼生殖过程中的一个关键阶段,为了明确这些激素对鳗鱼性成熟的影响,研究人员通过注射人类绒毛膜促性腺激素来诱导鳗鱼的性成熟。为了检测褪黑素和 DA 对性成熟的影响,以 1 毫克/千克体重的剂量向每组鱼腹腔注射 DA、褪黑素和 DA + 多潘立酮(每组 6 条)。然后使用定量 RT-PCR 技术,比较鳗鱼生殖相关基因(促性腺激素释放激素 1;gnrh1、促性腺激素释放激素 2;gnrh2、促卵泡激素;fshβ、黄体生成素;lhβ 和 DA 受体 2b;d2b)与对照组的信使 RNA 水平。结果表明,在性成熟的不同阶段,gnrh1、gnrh2和d2b在大脑中的表达水平以及d2b、fshβ、lhβ在垂体中的表达水平存在明显差异。褪黑激素似乎能促进促性腺激素的产生,而DA则会抑制它们。这些研究结果表明,褪黑激素和DA在调节日本鳗鱼的繁殖过程中存在相互作用。
Effects of dopamine and melatonin treatment on the expression of the genes associated with artificially induced sexual maturation in Japanese eel, Anguilla japonica
Japanese eel (Anguilla japonica) is a commercially important fish species in Asia. Understanding factors like photoperiod, temperature, and lunar cycles is crucial for successful aquaculture and managing its reproduction. Melatonin and dopamine (DA) are essential for regulating reproduction in vertebrates, including fish. This study investigated the effects of melatonin and DA on the reproductive system of mature male Japanese eels to better understand reproductive regulation in fish. To clarify the effects of these hormones on sexual maturation in eels, a critical stage in the reproductive process, sexual maturation was induced by injecting human chorionic gonadotropin, which stimulates the production of sex hormones. To check the effect of melatonin and DA on sexual maturation, DA, melatonin, and DA + domperidone were intraperitoneally injected into fish from each group (six per treatment) at a dose of 1 mg/kg body weight. The fish were then examined using quantitative RT-PCR by comparing the messenger RNA level of reproduction-related genes (gonadotropin releasing hormone 1; gnrh1, gonadotropin releasing hormone 2; gnrh2, follicle stimulating hormone; fshβ, luteinizing hormone; lhβ and DA receptor 2b; d2b), involved in the gonadotropic axis in eels, to those that received a control injection. The results indicate significant differences in the expression levels of gnrh1, gnrh2 and d2b in the brain and d2b, fshβ, lhβ in the pituitary at different stages of sexual maturation. Melatonin appears to enhance the production of sex gonadotropins, whereas DA inhibits them. These findings suggest an interaction between melatonin and DA in regulating reproduction in Japanese eels.
期刊介绍:
The Journal of Experimental Zoology – A publishes articles at the interface between Development, Physiology, Ecology and Evolution. Contributions that help to reveal how molecular, functional and ecological variation relate to one another are particularly welcome. The Journal publishes original research in the form of rapid communications or regular research articles, as well as perspectives and reviews on topics pertaining to the scope of the Journal. Acceptable articles are limited to studies on animals.