牛牦牛背长肌的全转录组分析揭示了 ADAMTS6 基因在肌母细胞中的调控功能。

IF 4.4 2区 化学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY ACS Applied Polymer Materials Pub Date : 2024-02-09 DOI:10.1016/j.ijbiomac.2024.129985
Chun Huang , Fen Feng , Rongfeng Dai , Wenwen Ren , Xinyi Li , Ta Zhaxi , Xiaoming Ma , Xiaoyun Wu , Min Chu , Yongfu La , Pengjia Bao , Xian Guo , Jie Pei , Ping Yan , Chunnian Liang
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引用次数: 0

摘要

牛牦牛是牛和牦牛的杂交 F1 代,与牦牛相比,牛牦牛具有更好的生产性能。然而,关于牛牦牛肌肉发育的分子机制的研究却很有限。针对这一知识空白,我们对牛牦牛背长肌进行了全面的转录组调查。研究表征了牛牦牛两个发育阶段(6 m、18 m)的三种转录本类型,即 lncRNA、miRNA 和 circRNA,以及蛋白质编码基因。研究结果表明,这些转录本在与成肌细胞分化和肌肉发育信号相关的通路中具有明显的富集作用。此外,该研究通过结合牦牛转录组数据并构建ceRNA共表达网络,发现了可能在牛牦牛肌肉发育过程中发挥关键作用的TCONS00024465/circHIPK3-bta-miR-499-ADAMTS6调控网络。利用 HEK 293 T 细胞验证了 TCONS00024465 和 circHIPK3 位于 bta-miR-499 的上游,并能作为 ceRNA 竞争性地与 bta-miR-499 结合。研究还验证了 ADAMTS6 通过抑制成肌细胞增殖、促进成肌细胞分化和正向调节成肌细胞凋亡来调控骨骼肌的发育。总之,这项研究为了解牛牦牛生产性能的优势提供了新的视角,并为进一步研究肌肉发育提供了分子基础。
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Whole-transcriptome analysis of longissimus dorsi muscle in cattle-yaks reveals the regulatory functions of ADAMTS6 gene in myoblasts

Cattle-yak, which is the hybrid F1 generation of cattle and yak, demonstrates better production performance compared to yak. However, there is limited research on the molecular mechanisms responsible for the muscle development of cattle-yak. To address this knowledge gap, a comprehensive transcriptomic survey of the longissimus dorsi muscle in cattle-yak was conducted. Three transcript types, namely lncRNAs, miRNAs, and circRNAs, along with protein-coding genes were characterized at two developmental stages (6 m, 18 m) of cattle-yak. The results revealed significant enrichment of these transcripts into pathways related to myoblast differentiation and muscle development signaling. Additionally, the study identified the TCONS00024465/circHIPK3-bta-miR-499-ADAMTS6 regulatory network, which may play a crucial role in the muscle development of cattle-yak by combining the transcriptome data of yak and constructing the ceRNA co-expression network. HEK 293 T cells were used to validate that TCONS00024465 and circHIPK3 are located upstream of bta-miR-499, and can competitively bind to bta-miR-499 as ceRNA. The study also verified that ADAMTS6 regulates skeletal muscle development by inhibiting myoblast proliferation, promoting myoblast differentiation, and positively regulating the apoptosis of myoblasts. Taken together, this study provides new insights into the advantages of cattle-yak production performance and offers a molecular basis for further research on muscle development.

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来源期刊
CiteScore
7.20
自引率
6.00%
发文量
810
期刊介绍: ACS Applied Polymer Materials is an interdisciplinary journal publishing original research covering all aspects of engineering, chemistry, physics, and biology relevant to applications of polymers. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates fundamental knowledge in the areas of materials, engineering, physics, bioscience, polymer science and chemistry into important polymer applications. The journal is specifically interested in work that addresses relationships among structure, processing, morphology, chemistry, properties, and function as well as work that provide insights into mechanisms critical to the performance of the polymer for applications.
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