微型设备上细菌肠道病原体的快速检测:概述。

IF 3.9 3区 医学 Q1 PATHOLOGY Expert Review of Molecular Diagnostics Pub Date : 2024-03-01 Epub Date: 2024-02-13 DOI:10.1080/14737159.2024.2316756
Gratiela Gradisteanu Pircalabioru, Mina Raileanu, Mihai Viorel Dionisie, Irina-Oana Lixandru-Petre, Ciprian Iliescu
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引用次数: 0

摘要

导言:肠道微生物带来了结肠发炎、致命腹泻、抗菌药耐药性传播和慢性病发病等挑战。开发早期、快速和特异性诊断工具对于改善感染控制至关重要。床旁检测(POCT)系统为从各种临床和环境样本中检测微生物提供了快速、灵敏、低成本和从样本到答案的方法,具有便携、自动化和操作简单等优点:肠道微生物的快速检测可采用多种技术,包括生物传感器、免疫测定、电化学阻抗光谱、质谱分析和分子生物学。纳入物联网、机器学习和基于智能手机的护理点应用是 POCT 的一个重要方面。在这篇综述中,作者讨论了各种肠道病原体快速诊断平台及其主要挑战:开发有效的微生物检测方法可能很复杂。检测设计必须考虑目标选择、实时和多重检测、样本类型、试剂稳定性和储存、引物/探针设计以及优化反应条件以提高准确性和灵敏度等因素。应对这些挑战需要科学家、临床医生、工程师和行业合作伙伴之间的跨学科合作。未来的努力对于提高用于肠道微生物检测和定量的 POCT 系统的灵敏度、特异性和多功能性,推进传染病诊断和管理至关重要。
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Fast detection of bacterial gut pathogens on miniaturized devices: an overview.

Introduction: Gut microbes pose challenges like colon inflammation, deadly diarrhea, antimicrobial resistance dissemination, and chronic disease onset. Development of early, rapid and specific diagnosis tools is essential for improving infection control. Point-of-care testing (POCT) systems offer rapid, sensitive, low-cost and sample-to-answer methods for microbe detection from various clinical and environmental samples, bringing the advantages of portability, automation, and simple operation.

Areas covered: Rapid detection of gut microbes can be done using a wide array of techniques including biosensors, immunological assays, electrochemical impedance spectroscopy, mass spectrometry and molecular biology. Inclusion of Internet of Things, machine learning, and smartphone-based point-of-care applications is an important aspect of POCT. In this review, the authors discuss various fast diagnostic platforms for gut pathogens and their main challenges.

Expert opinion: Developing effective assays for microbe detection can be complex. Assay design must consider factors like target selection, real-time and multiplex detection, sample type, reagent stability and storage, primer/probe design, and optimizing reaction conditions for accuracy and sensitivity. Mitigating these challenges requires interdisciplinary collaboration among scientists, clinicians, engineers, and industry partners. Future efforts are essential to enhance sensitivity, specificity, and versatility of POCT systems for gut microbe detection and quantification, advancing infectious disease diagnostics and management.

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来源期刊
CiteScore
6.60
自引率
0.00%
发文量
71
审稿时长
1 months
期刊介绍: Expert Review of Molecular Diagnostics (ISSN 1473-7159) publishes expert reviews of the latest advancements in the field of molecular diagnostics including the detection and monitoring of the molecular causes of disease that are being translated into groundbreaking diagnostic and prognostic technologies to be used in the clinical diagnostic setting. Each issue of Expert Review of Molecular Diagnostics contains leading reviews on current and emerging topics relating to molecular diagnostics, subject to a rigorous peer review process; editorials discussing contentious issues in the field; diagnostic profiles featuring independent, expert evaluations of diagnostic tests; meeting reports of recent molecular diagnostics conferences and key paper evaluations featuring assessments of significant, recently published articles from specialists in molecular diagnostic therapy. Expert Review of Molecular Diagnostics provides the forum for reporting the critical advances being made in this ever-expanding field, as well as the major challenges ahead in their clinical implementation. The journal delivers this information in concise, at-a-glance article formats: invaluable to a time-constrained community.
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