细胞中的生物医学应用、前景和标签设计概念 - 无声拉曼窗口

IF 4.2 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY RSC Chemical Biology Pub Date : 2024-02-12 DOI:10.1039/D3CB00217A
Martha Z. Vardaki, Vasilis G. Gregoriou and Christos L. Chochos
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引用次数: 0

摘要

光谱研究越来越多地采用在拉曼光谱的细胞静默区(1800-2800 cm-1)显示信号的拉曼标签,因为该区域本来就没有生物分子产生的波段。带有包含三键(如炔烃键、腈键或碳-氘键)的官能团的拉曼标记在该区域有明显的振动频率。由于在特定区域没有光谱背景和细胞相关带,使用这些标签有助于克服生物样本测量中信噪比差和存在重叠拉曼带的固有问题。细胞静默拉曼标签可对生物分子进行生物正交成像,提高化学对比度,并已在分析物检测和监测、生物标记分析和活细胞成像中得到应用。本综述将重点关注细胞无声拉曼区域的潜力,报告利用拉曼光谱变体在生物医学应用中使用的标签。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Biomedical applications, perspectives and tag design concepts in the cell – silent Raman window†

Spectroscopic studies increasingly employ Raman tags exhibiting a signal in the cell – silent region of the Raman spectrum (1800–2800 cm−1), where bands arising from biological molecules are inherently absent. Raman tags bearing functional groups which contain a triple bond, such as alkyne and nitrile or a carbon–deuterium bond, have a distinct vibrational frequency in this region. Due to the lack of spectral background and cell-associated bands in the specific area, the implementation of those tags can help overcome the inherently poor signal-to-noise ratio and presence of overlapping Raman bands in measurements of biological samples. The cell – silent Raman tags allow for bioorthogonal imaging of biomolecules with improved chemical contrast and they have found application in analyte detection and monitoring, biomarker profiling and live cell imaging. This review focuses on the potential of the cell – silent Raman region, reporting on the tags employed for biomedical applications using variants of Raman spectroscopy.

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CiteScore
6.10
自引率
0.00%
发文量
128
审稿时长
10 weeks
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