Ge Diao, Min Tian, Runbo Li, Jie Huang, Jian Han, Jianxin Guo
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The genomic copy number determination of improved Cre in transgenic mice using standards derived from megaprimer PCR constructed tandem repeats
Determination of the genomic copy number of Cre transgene is critical for the establishment of transgenic animal models. We demonstrate a real-time quantitative polymerase chain reaction (RT-qPCR) ...
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Frontiers in Life Science publishes high quality and innovative research at the frontier of biology with an emphasis on interdisciplinary research. We particularly encourage manuscripts that lie at the interface of the life sciences and either the more quantitative sciences (including chemistry, physics, mathematics, and informatics) or the social sciences (philosophy, anthropology, sociology and epistemology). We believe that these various disciplines can all contribute to biological research and provide original insights to the most recurrent questions.
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