炎症性肠病患者血清 Zonulin 与先天性免疫之间的关系

G. Khusainova, V. Genkel, Alla Kuznetsova, K. Nikushkina, A. Saenko, Olga Abramovskikh, Anastasiya Dolgushina
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摘要

这项研究的目的是调查溃疡性结肠炎(UC)和克罗恩病(CD)患者血清zonulin 与细胞免疫之间的关系。研究共纳入 97 名患者,其中 13 名(13.4%)为 CD 患者,84 名(86.6%)为 UC 患者。使用酶联免疫吸附试剂盒研究了血清中唑蛋白的浓度。通过流式细胞术对循环白细胞亚群进行了调查。利用单分散聚苯乙烯乳胶颗粒的吸收能力和硝基蓝四氮唑试验中的氧依赖性代谢指标研究了循环中性粒细胞的功能活性。与 UC 患者相比,CD 患者的血清 zonulin 浓度明显更高(p = 0.003)。zonulin 浓度与循环中性粒细胞的功能活性直接相关。zonulin浓度大于472.4 pg/mL的患者,其带状中性粒细胞(p = 0.0104)、CD3+CD8+细胞(p = 0.0212)、NK细胞(p = 0.0161)和低CD19+细胞(p = 0.0034)的数量明显较多。在 IBD 患者中,zonulin 与 IBD 的严重程度有关。血清中 zonulin 浓度的增加与循环中性粒细胞功能活性的增加、CD3+CD8+细胞和 NK 细胞数量的增加以及 CD19+ 细胞数量的减少有关。
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The Relationship between Serum Zonulin and Innate Immunity in Patients with Inflammatory Bowel Disease
The aim of the study was to investigate the relationship between the serum zonulin and the cellular immunity in patients with ulcerative colitis (UC) and Crohn’s disease (CD). The study included 97 patients, 13 (13.4%) patients with CD and 84 (86.6%) patients with UC. The concentration of zonulin in the serum was studied using the ELISA kits. The investigation of the circulated leukocyte subpopulation was carried out through flow cytometry. The functional activity of the circulating neutrophils was studied using the absorption capacity of the monodisperse polystyrene latex particles and indicators of oxygen-dependent metabolism in the nitroblue tetrazolium test. The serum zonulin concentration in CD patients was significantly higher compared with UC patients (p = 0.003). The zonulin concentration directly correlated with the functional activity of the circulating neutrophils. Patients with a zonulin concentration > 472.4 pg/mL had a significantly higher number of band neutrophils (p = 0.0104), CD3+CD8+ cells (p = 0.0212), NK cells (p = 0.0161), and lower–CD19+ cells (p = 0.0034). Among the IBD patients, zonulin was associated with IBD severity. An increase in the serum concentration of zonulin was associated with an increase in the functional activity of circulating neutrophils and an increase in the number of CD3+CD8+ cells, NK cells, and a decrease in the number of CD19+ cells.
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