Atikah D. Putri, Varsha Singh, E. D. De Castro, C. A. Rutland, J. Scott McElroy, Te-ming Tseng, J. McCurdy
{"title":"确认平滑蟹草(Digitaria ischaemum)对喹啉酸的抗性及其代谢差异","authors":"Atikah D. Putri, Varsha Singh, E. D. De Castro, C. A. Rutland, J. Scott McElroy, Te-ming Tseng, J. McCurdy","doi":"10.1017/wsc.2024.6","DOIUrl":null,"url":null,"abstract":"\n Quinclorac controls crabgrass (Digitaria spp.) in cool- and warm-season turfgrass species. Herbicide-resistant smooth crabgrass [Digitaria ischaemum (Schreb.) Schreb. Ex Muhl.]D. ischaemum biotypes have evolved due to recurrent usage of quinclorac. Two Mississippi populations (MSU1 and MSU2) of D. ischaemum were characterized using standard greenhouse dose-response screens in order to assess their resistance relative to known susceptible populations. Subsequent investigations explored mechanisms of resistance, including examining cyanide accumulation, glutathione-S-transferase (GST) activity, and the potential involvement of cytochrome P450s in MSU1, MSU2, and a susceptible (SMT2). Resistant populations MSU1 and MSU2 required 80 and five times more quinclorac, respectively, to reach 50% biomass reduction than susceptible populations. The SMT2 biotype accumulated three times more cyanide than the resistant MSU1 and MSU2 populations. GST activity was elevated in resistant MSU1 and MSU2 populations. Furthermore, quinclorac concentrations of treated resistant populations was elevated when plants were pre-treated with the P450 inhibitor malathion. These findings suggest a non-target-site–based mechanism of resistance involving the accumulation of cyanide. This may provide a scientific basis for understanding the occurrence of quinclorac-resistant D. ischaemum, although further research is needed to investigate potential target-site mechanisms of resistance.","PeriodicalId":23688,"journal":{"name":"Weed Science","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Confirmation and Differential Metabolism Associated with Quinclorac Resistance in Smooth Crabgrass (Digitaria ischaemum)\",\"authors\":\"Atikah D. Putri, Varsha Singh, E. D. De Castro, C. A. Rutland, J. Scott McElroy, Te-ming Tseng, J. McCurdy\",\"doi\":\"10.1017/wsc.2024.6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n Quinclorac controls crabgrass (Digitaria spp.) in cool- and warm-season turfgrass species. Herbicide-resistant smooth crabgrass [Digitaria ischaemum (Schreb.) Schreb. Ex Muhl.]D. ischaemum biotypes have evolved due to recurrent usage of quinclorac. Two Mississippi populations (MSU1 and MSU2) of D. ischaemum were characterized using standard greenhouse dose-response screens in order to assess their resistance relative to known susceptible populations. Subsequent investigations explored mechanisms of resistance, including examining cyanide accumulation, glutathione-S-transferase (GST) activity, and the potential involvement of cytochrome P450s in MSU1, MSU2, and a susceptible (SMT2). Resistant populations MSU1 and MSU2 required 80 and five times more quinclorac, respectively, to reach 50% biomass reduction than susceptible populations. The SMT2 biotype accumulated three times more cyanide than the resistant MSU1 and MSU2 populations. GST activity was elevated in resistant MSU1 and MSU2 populations. Furthermore, quinclorac concentrations of treated resistant populations was elevated when plants were pre-treated with the P450 inhibitor malathion. These findings suggest a non-target-site–based mechanism of resistance involving the accumulation of cyanide. This may provide a scientific basis for understanding the occurrence of quinclorac-resistant D. ischaemum, although further research is needed to investigate potential target-site mechanisms of resistance.\",\"PeriodicalId\":23688,\"journal\":{\"name\":\"Weed Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-02-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Weed Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1017/wsc.2024.6\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Weed Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1017/wsc.2024.6","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
Confirmation and Differential Metabolism Associated with Quinclorac Resistance in Smooth Crabgrass (Digitaria ischaemum)
Quinclorac controls crabgrass (Digitaria spp.) in cool- and warm-season turfgrass species. Herbicide-resistant smooth crabgrass [Digitaria ischaemum (Schreb.) Schreb. Ex Muhl.]D. ischaemum biotypes have evolved due to recurrent usage of quinclorac. Two Mississippi populations (MSU1 and MSU2) of D. ischaemum were characterized using standard greenhouse dose-response screens in order to assess their resistance relative to known susceptible populations. Subsequent investigations explored mechanisms of resistance, including examining cyanide accumulation, glutathione-S-transferase (GST) activity, and the potential involvement of cytochrome P450s in MSU1, MSU2, and a susceptible (SMT2). Resistant populations MSU1 and MSU2 required 80 and five times more quinclorac, respectively, to reach 50% biomass reduction than susceptible populations. The SMT2 biotype accumulated three times more cyanide than the resistant MSU1 and MSU2 populations. GST activity was elevated in resistant MSU1 and MSU2 populations. Furthermore, quinclorac concentrations of treated resistant populations was elevated when plants were pre-treated with the P450 inhibitor malathion. These findings suggest a non-target-site–based mechanism of resistance involving the accumulation of cyanide. This may provide a scientific basis for understanding the occurrence of quinclorac-resistant D. ischaemum, although further research is needed to investigate potential target-site mechanisms of resistance.
期刊介绍:
Weed Science publishes original research and scholarship in the form of peer-reviewed articles focused on fundamental research directly related to all aspects of weed science in agricultural systems. Topics for Weed Science include:
- the biology and ecology of weeds in agricultural, forestry, aquatic, turf, recreational, rights-of-way and other settings, genetics of weeds
- herbicide resistance, chemistry, biochemistry, physiology and molecular action of herbicides and plant growth regulators used to manage undesirable vegetation
- ecology of cropping and other agricultural systems as they relate to weed management
- biological and ecological aspects of weed control tools including biological agents, and herbicide resistant crops
- effect of weed management on soil, air and water.