多糖胶囊及其庚糖对空肠弯曲杆菌抵抗先天性免疫防御的影响。

IF 3.9 3区 生物学 Q2 MICROBIOLOGY MicrobiologyOpen Pub Date : 2024-02-20 DOI:10.1002/mbo3.1400
Matthew Myles, Heba Barnawi, Mahmoud Mahmoudpour, Sargon Shlimon, Adrienne Chang, Daniel Zimmermann, Chiwon Choi, Najwa Zebian, Carole Creuzenet
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引用次数: 0

摘要

空肠弯曲菌是许多动物的共生菌,但会导致人类腹泻。其多糖胶囊以菌株和模型特异性的方式促进宿主定植和毒力。我们研究了多糖胶囊及其庚糖对 NCTC 11168 株与各种宿主及其先天免疫防御系统的相互作用是否重要。我们确定,它们支持细菌在黑腹果蝇中的存活,并增强了其在黑腹果蝇中的毒力。我们发现,在人和鸡的巨噬细胞中,胶囊的抗虹吸活性有限,对鸡巨噬细胞的粘附性降低,两种巨噬细胞的细胞内存活率降低。与此相反,七糖增加了鸡巨噬细胞的吸收,并支持人巨噬细胞的粘附和在其中存活。鸡巨噬细胞中的胶囊会引发一氧化氮的产生,而七聚糖则会减轻这种情况,并保护巨噬细胞免受亚硝酸攻击。最后,空肠大肠杆菌菌株 NCTC 11168 在两种巨噬细胞中都能引起强烈的细胞因子产生,但能独立于胶囊和庚糖淬灭 ROS 的产生,虽然胶囊和庚糖不能抵御氧化攻击,但它们有利于氧化应激下生物膜的生长。这项研究表明,野生型胶囊及其庚糖往往通过胶囊及其庚糖的拮抗作用,优化了菌株 NCTC 11168 抵抗先天防御的能力。
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Effect of the polysaccharide capsule and its heptose on the resistance of Campylobacter jejuni to innate immune defenses

Campylobacter jejuni is a commensal in many animals but causes diarrhea in humans. Its polysaccharide capsule contributes to host colonization and virulence in a strain- and model-specific manner. We investigated if the capsule and its heptose are important for interactions of strain NCTC 11168 with various hosts and their innate immune defenses. We determined that they support bacterial survival in Drosophila melanogaster and enhance virulence in Galleria mellonella. We showed that the capsule had limited antiphagocytic activity in human and chicken macrophages, decreased adherence to chicken macrophages, and decreased intracellular survival in both macrophages. In contrast, the heptose increased uptake by chicken macrophages and supported adherence to human macrophages and survival within them. While the capsule triggered nitric oxide production in chicken macrophages, the heptose mitigated this and protected against nitrosative assault. Finally, the C. jejuni strain NCTC 11168 elicited strong cytokine production in both macrophages but quenched ROS production independently from capsule and heptose, and while the capsule and heptose did not protect against oxidative assault, they favored growth in biofilms under oxidative stress. This study shows that the wild-type capsule with its heptose is optimized to resist innate defenses in strain NCTC 11168 often via antagonistic effects of the capsule and its heptose.

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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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