用于检测苹果和梨上卷曲病毒属(包括检疫物种 V. nashicola)的物种特异性实时 PCR 检测方法

IF 2.2 4区 农林科学 Q2 PLANT SCIENCES Journal of Plant Pathology Pub Date : 2024-02-22 DOI:10.1007/s42161-024-01615-1
Elodie Muller, Jason Shiller, Bruno Le Cam, Yohana Laloum, Michel Girault, Renaud Ioos, Cécile Guinet
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引用次数: 0

摘要

Venturia spp.的几个种类会引起果树疮痂病:苹果上的 V. inaequalis、梨上的 V. pirina 和亚洲梨上的 V. nashicola 被世界上多个国家列为检疫病原体。最近,法国、意大利和中国报告了一种由苹果上的 V. asperata 引起的新病害。果树疮痂病会造成很高的经济损失,果园需要经常使用杀菌剂。对这些病原体的早期检测对于管理这种病害非常重要,而对于 V. nashicola 来说,则可以防止其在无病害地区的引入和传播。利用这些物种的基因组资源,我们确定了它们之间的多态区,从而开发出一套实时 PCR 检测方法,能够在有症状的果实和叶片上检测到这四个物种。我们特别关注 V. nashicola,以建立一个全面的验证程序。事实证明,该检测方法可以有效地针对这一检疫物种,从而确保在监管监测中分析结果的可靠性。
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Species-specific real-time PCR assays for the detection of Venturia spp. on apple and pear, including the quarantine species V. nashicola

Several species of Venturia spp. cause scab disease on fruit trees: V. inaequalis on apple, V. pirina on pear, and V. nashicola on Asian pear that is listed as a quarantine pathogen in several countries in the world. An emerging disease caused by V. asperata on apple has very recently been reported in France, Italy and China. Fruit tree scab causes high economic losses and requires frequent fungicide treatments in orchards. Early detection of these pathogens is important in the management of this disease and—in the case of V. nashicola—to prevent its introduction and spread in disease-free areas. Using genomic resources available on these species, we identified polymorphic regions between them to develop a set of real-time PCR assays enabling detection of the four species on symptomatic fruits and leaves. We focused in particular on V. nashicola to establish a comprehensive validation procedure. The assay proved to be effective for targeting this quarantine species, thereby ensuring the reliability of analysis results in the context of regulatory monitoring.

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来源期刊
Journal of Plant Pathology
Journal of Plant Pathology 生物-植物科学
CiteScore
3.10
自引率
4.50%
发文量
218
审稿时长
6-12 weeks
期刊介绍: The Journal of Plant Pathology (JPP or JPPY) is the main publication of the Italian Society of Plant Pathology (SiPAV), and publishes original contributions in the form of full-length papers, short communications, disease notes, and review articles on mycology, bacteriology, virology, phytoplasmatology, physiological plant pathology, plant-pathogeninteractions, post-harvest diseases, non-infectious diseases, and plant protection. In vivo results are required for plant protection submissions. Varietal trials for disease resistance and gene mapping are not published in the journal unless such findings are already employed in the context of strategic approaches for disease management. However, studies identifying actual genes involved in virulence are pertinent to thescope of the Journal and may be submitted. The journal highlights particularly timely or novel contributions in its Editors’ choice section, to appear at the beginning of each volume. Surveys for diseases or pathogens should be submitted as "Short communications".
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