Cheng Li, Hancheng Zhou, Huiqing Gou, Zixin Fan, Yifei Zhang, Pengzhou Tang, Jiaxin Huang, Yan Xu, Lu Li
{"title":"牙周炎中口腔微生物菌群产生的自身诱导因子-2与牙槽骨流失。","authors":"Cheng Li, Hancheng Zhou, Huiqing Gou, Zixin Fan, Yifei Zhang, Pengzhou Tang, Jiaxin Huang, Yan Xu, Lu Li","doi":"10.1111/jre.13247","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>The aim of this study was to investigate the association between autoinducer-2 (AI-2) of oral microbial flora and the alveolar bone destruction in periodontitis to determine if AI-2 may have the potential that monitor periodontitis and predict bone loss.</p>\n </section>\n \n <section>\n \n <h3> Background</h3>\n \n <p>Plaque biofilm was the initiating factor of periodontitis and the essential factor of periodontal tissue destruction. The formation of biofilms depended on the complex regulation of the quorum sensing (QS) system, in which bacteria could sense changes in surrounding bacterial density by secreting the autoinducer (AI) to regulate the corresponding physiological function. Most oral bacteria also communicated with each other to form biofilms administrating the QS system, which implied that the QS system of periodontal pathogens was related to periodontitis, but the specific relationship was unknown.</p>\n </section>\n \n <section>\n \n <h3> Method</h3>\n \n <p>We collected the gingival crevicular fluid (GCF) samples and measured the concentration of AI-2 in samples using the <i>Vibrio harveyi</i> BB180 bioluminescent-reporter system. To explore the interaction between AI-2 and bone metabolism, we utilized AI-2 purified from <i>Fusobacterium nucleatum</i> to investigate the impact of <i>F. nucleatum</i> AI-2 on osteoclast differentiation. Moreover, we constructed murine periodontitis models and multi-species biofilm models to study the association between AI-2 and periodontal disease progression.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The AI-2 concentration in GCF samples increased along with periodontal disease progression (<i>p</i> < .0001). <i>F. nucleatum</i> AI-2 promoted osteoclast differentiation in a dose-dependent manner. In the periodontitis mice model, the CEJ-ABC distance in the <i>F. nucleatum</i> AI-2 treatment group was higher than that in the simple ligation group (<i>p</i> < .01), and the maxilla of the mice in the group exhibited significantly lower BMD and BV/TV values (<i>p</i> < .05).</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>We demonstrated that the AI-2 concentration varied with the alveolar bone destruction in periodontitis, and it may have the potential for screening periodontitis. <i>F. nucleatum</i> AI-2 promoted osteoclast differentiation in a dose-dependent manner and aggravated bone loss.</p>\n </section>\n </div>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Autoinducer-2 produced by oral microbial flora and alveolar bone loss in periodontitis\",\"authors\":\"Cheng Li, Hancheng Zhou, Huiqing Gou, Zixin Fan, Yifei Zhang, Pengzhou Tang, Jiaxin Huang, Yan Xu, Lu Li\",\"doi\":\"10.1111/jre.13247\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>The aim of this study was to investigate the association between autoinducer-2 (AI-2) of oral microbial flora and the alveolar bone destruction in periodontitis to determine if AI-2 may have the potential that monitor periodontitis and predict bone loss.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Plaque biofilm was the initiating factor of periodontitis and the essential factor of periodontal tissue destruction. The formation of biofilms depended on the complex regulation of the quorum sensing (QS) system, in which bacteria could sense changes in surrounding bacterial density by secreting the autoinducer (AI) to regulate the corresponding physiological function. Most oral bacteria also communicated with each other to form biofilms administrating the QS system, which implied that the QS system of periodontal pathogens was related to periodontitis, but the specific relationship was unknown.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Method</h3>\\n \\n <p>We collected the gingival crevicular fluid (GCF) samples and measured the concentration of AI-2 in samples using the <i>Vibrio harveyi</i> BB180 bioluminescent-reporter system. To explore the interaction between AI-2 and bone metabolism, we utilized AI-2 purified from <i>Fusobacterium nucleatum</i> to investigate the impact of <i>F. nucleatum</i> AI-2 on osteoclast differentiation. Moreover, we constructed murine periodontitis models and multi-species biofilm models to study the association between AI-2 and periodontal disease progression.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The AI-2 concentration in GCF samples increased along with periodontal disease progression (<i>p</i> < .0001). <i>F. nucleatum</i> AI-2 promoted osteoclast differentiation in a dose-dependent manner. In the periodontitis mice model, the CEJ-ABC distance in the <i>F. nucleatum</i> AI-2 treatment group was higher than that in the simple ligation group (<i>p</i> < .01), and the maxilla of the mice in the group exhibited significantly lower BMD and BV/TV values (<i>p</i> < .05).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>We demonstrated that the AI-2 concentration varied with the alveolar bone destruction in periodontitis, and it may have the potential for screening periodontitis. <i>F. nucleatum</i> AI-2 promoted osteoclast differentiation in a dose-dependent manner and aggravated bone loss.</p>\\n </section>\\n </div>\",\"PeriodicalId\":16715,\"journal\":{\"name\":\"Journal of periodontal research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-02-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of periodontal research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jre.13247\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of periodontal research","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jre.13247","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Autoinducer-2 produced by oral microbial flora and alveolar bone loss in periodontitis
Objective
The aim of this study was to investigate the association between autoinducer-2 (AI-2) of oral microbial flora and the alveolar bone destruction in periodontitis to determine if AI-2 may have the potential that monitor periodontitis and predict bone loss.
Background
Plaque biofilm was the initiating factor of periodontitis and the essential factor of periodontal tissue destruction. The formation of biofilms depended on the complex regulation of the quorum sensing (QS) system, in which bacteria could sense changes in surrounding bacterial density by secreting the autoinducer (AI) to regulate the corresponding physiological function. Most oral bacteria also communicated with each other to form biofilms administrating the QS system, which implied that the QS system of periodontal pathogens was related to periodontitis, but the specific relationship was unknown.
Method
We collected the gingival crevicular fluid (GCF) samples and measured the concentration of AI-2 in samples using the Vibrio harveyi BB180 bioluminescent-reporter system. To explore the interaction between AI-2 and bone metabolism, we utilized AI-2 purified from Fusobacterium nucleatum to investigate the impact of F. nucleatum AI-2 on osteoclast differentiation. Moreover, we constructed murine periodontitis models and multi-species biofilm models to study the association between AI-2 and periodontal disease progression.
Results
The AI-2 concentration in GCF samples increased along with periodontal disease progression (p < .0001). F. nucleatum AI-2 promoted osteoclast differentiation in a dose-dependent manner. In the periodontitis mice model, the CEJ-ABC distance in the F. nucleatum AI-2 treatment group was higher than that in the simple ligation group (p < .01), and the maxilla of the mice in the group exhibited significantly lower BMD and BV/TV values (p < .05).
Conclusions
We demonstrated that the AI-2 concentration varied with the alveolar bone destruction in periodontitis, and it may have the potential for screening periodontitis. F. nucleatum AI-2 promoted osteoclast differentiation in a dose-dependent manner and aggravated bone loss.
期刊介绍：
The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published.
One volume of six issues is published annually.
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