与口腔增生性结节有关的北部海獭Enhydra lutris kenyoni体内的新型羊羔病毒。

IF 1.1 4区 农林科学 Q3 FISHERIES Diseases of aquatic organisms Pub Date : 2024-02-29 DOI:10.3354/dao03771
Carlos H Romero, Pam Tuomi, Kathleen A Burek-Huntington, Verena A Gill
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引用次数: 0

摘要

研究人员对 2004 年在美国阿拉斯加霍默发现的一种新型乳头状瘤病毒(PV)进行了基因鉴定,这种病毒与一只死亡的野生亚成年北海獭(Enhydra lutris kenyoni,NSO)口腔黏膜-皮肤边界散布的增生结节有关。设计了用于扩增包含 NSO PV 完整基因组的 2 个大的重叠 DNA 片段的引物。桑格方法产生了序列,并据此设计了新的特异引物,用于引物游走法。NSO PV基因组由8085个核苷酸组成,包含一个由E6、E7、E1和E2开放阅读框(ORF)组成的早期区域、一个缺乏框内近端ATG起始密码子的E4 ORF(包含在E2中)、一个缺乏任何ORF的异常长(907个核苷酸)区段、一个包含帽状体基因L2和L1的晚期区域以及一个非编码调控区(ncRR)。这种 NSO PV 被暂时命名为 Enhydra lutris kenyoni PV2(ElkPV2)。完整的 L1 ORF 核苷酸和 E1-E2-L1 氨基酸序列的配对和多重序列比对显示,NSO PV 是一种新型 PV,在系统发育上与南部海獭 PV1 的亲缘关系最为密切。E6 肿瘤蛋白的羧基末端不包含与致癌性密切相关的 PDZ 结合基序,这表明这是一种低风险的 PV,这与组织病理学结果一致。然而,ElkPV2 E7 肿瘤蛋白确实含有视网膜母细胞瘤(pRb)结合结构域 LXCXE(ElkPV2 中为 LQCYE),这与某些高风险 PV 的致癌性有关。我们需要进一步研究 ElkPV2 在 NSO 中的感染率和临床意义。
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Novel lambdapapillomavirus in northern sea otters Enhydra lutris kenyoni, associated with oral hyperplastic nodules.

A novel papillomavirus (PV) associated with hyperplastic nodules scattered over the muco-cutaneous border of the oral cavity of a dead, wild, subadult northern sea otter Enhydra lutris kenyoni (NSO) in 2004 in Homer, Alaska, USA, was genetically characterized. Primers for the amplification of 2 large overlapping DNA fragments that contained the complete genome of the NSO PV were designed. Sanger methodology generated sequences from which new specific primers were designed for the primer-walking approach. The NSO PV genome consists of 8085 nucleotides and contains an early region composed of E6, E7, E1, and E2 open reading frames (ORFs), an E4 ORF (contained within E2) lacking an in-frame proximal ATG start codon, an unusually long (907 nucleotide) stretch lacking any ORFs, a late region that contains the capsid genes L2 and L1, and a non-coding regulatory region (ncRR). This NSO PV has been tentatively named Enhydra lutris kenyoni PV2 (ElkPV2). Pairwise and multiple sequence alignments of the complete L1 ORF nucleotides and concatenated E1-E2-L1 amino acid sequences showed that the NSO PV is a novel PV, phylogenetically most closely related to southern sea otter PV1. The carboxy end of the E6 oncoprotein does not contain the PDZ-binding motif with a strong correlation with oncogenicity, suggesting a low-risk PV, which is in agreement with histopathological findings. However, the ElkPV2 E7 oncoprotein does contain the retinoblastoma (pRb) binding domain LXCXE (LQCYE in ElkPV2), associated with oncogenicity in some high-risk PVs. Further studies on the prevalence and clinical significance of ElkPV2 infections in NSO are needed.

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来源期刊
Diseases of aquatic organisms
Diseases of aquatic organisms 农林科学-兽医学
CiteScore
3.10
自引率
0.00%
发文量
53
审稿时长
8-16 weeks
期刊介绍: DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically: -Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens -Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)- Diseases due to internal circumstances (innate, idiopathic, genetic)- Diseases due to proliferative disorders (neoplasms)- Disease diagnosis, treatment and prevention- Molecular aspects of diseases- Nutritional disorders- Stress and physical injuries- Epidemiology/epizootiology- Parasitology- Toxicology- Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)- Diseases as indicators of humanity''s detrimental impact on nature- Genomics, proteomics and metabolomics of disease- Immunology and disease prevention- Animal welfare- Zoonosis
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