染色盒家族成员对人类前列腺癌的预测意义。

IF 4.9 2区 医学 Q2 CELL BIOLOGY Cellular Oncology Pub Date : 2024-08-01 Epub Date: 2024-03-01 DOI:10.1007/s13402-024-00929-7
Xiaoting Xu, Cong Lai, Jiawen Luo, Juanyi Shi, Kaixuan Guo, Jintao Hu, Yelisudan Mulati, Yunfei Xiao, Degeng Kong, Cheng Liu, Jingang Huang, Kewei Xu
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引用次数: 0

摘要

目的:Chromobox(CBX)家族蛋白是表观遗传调控机制的关键元素,在癌症的发生和发展中发挥着重要作用。然而,人们对 CBX 在前列腺癌(PCa)的发生和发展中的作用了解有限。我们的目标是建立一个与 CBXs 相关的独特预后模型,以提高预测 PCa 患者预后的准确性:方法:分析来自 TCGA 和 GEO 数据库的数据,以评估差异表达、预后价值、基因通路富集和免疫细胞浸润。利用 COX 回归分析确定影响无病生存期(DFS)的独立预后因素。临床组织切片的免疫组化染色验证了 CBX2 的表达和 FOXP3+ 细胞的浸润。体外增殖、迁移和侵袭试验检验了 CBX2 的功能。采用RNA-seq技术检测CBX2相关通路的富集情况:结果:在PCa组织中,CBX2、CBX3、CBX4和CBX8上调,而CBX6和CBX7下调。CBXs的表达因分期和分级而异。CBX1、CBX2、CBX3、CBX4和CBX8的表达升高与预后不良有关。CBX2的表达、T期和Gleason评分是独立的预后因素。CBX2 在 PCa 组织中的表达水平明显高于邻近的正常组织。在CBX2高表达组中观察到更多的Treg浸润。CBX2的表达影响了PCa细胞的生长、迁移和侵袭:CBX2参与了PCa的发生和发展,表明它有可能成为PCa患者的可靠预后指标。
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The predictive significance of chromobox family members in prostate cancer in humans.

Purpose: The Chromobox (CBX) family proteins are crucial elements of the epigenetic regulatory machinery and play a significant role in the development and advancement of cancer. Nevertheless, there is limited understanding regarding the role of CBXs in development or progression of prostate cancer (PCa). Our objective is to develop a unique prognostic model associated with CBXs to improve the accuracy of predicting outcomes of patients with PCa.

Methods: Data from TCGA and GEO databases were analyzed to assess differential expression, prognostic value, gene pathway enrichment, and immune cell infiltration. COX regression analysis was utilized to identify the independent prognostic factors that impact disease-free survival (DFS). The expression of CBX2 and FOXP3+ cells infiltration was verified by immunohistochemical staining of clinical tissue sections. In vitro proliferation, migration and invasion assay were conducted to examine the function of CBX2. RNA-seq was employed to examine the CBX2 related pathway enrichment.

Results: CBX2, CBX3, CBX4, and CBX8 were upregulated, while CBX6 and CBX7 were downregulated in PCa tissues. CBXs expression varied by stage and grade. Elevated expression of CBX1, CBX2, CBX3, CBX4 and CBX8 is correlated with poor outcome. CBX2 expression, T stage, and Gleason score were independent prognostic factors. The expression level of CBX2 in PCa tissues was significantly higher than that in adjacent normal tissues. More Treg infiltration was observed in the group with high CBX2 expression. CBX2 expression affected PCa cell growth, migration, and invasion.

Conclusions: CBX2 is involved in the development and advancement of PCa, suggesting its potential as a reliable prognostic indicator for PCa patients.

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来源期刊
Cellular Oncology
Cellular Oncology ONCOLOGY-CELL BIOLOGY
CiteScore
10.30
自引率
1.50%
发文量
86
审稿时长
12 months
期刊介绍: The Official Journal of the International Society for Cellular Oncology Focuses on translational research Addresses the conversion of cell biology to clinical applications Cellular Oncology publishes scientific contributions from various biomedical and clinical disciplines involved in basic and translational cancer research on the cell and tissue level, technical and bioinformatics developments in this area, and clinical applications. This includes a variety of fields like genome technology, micro-arrays and other high-throughput techniques, genomic instability, SNP, DNA methylation, signaling pathways, DNA organization, (sub)microscopic imaging, proteomics, bioinformatics, functional effects of genomics, drug design and development, molecular diagnostics and targeted cancer therapies, genotype-phenotype interactions. A major goal is to translate the latest developments in these fields from the research laboratory into routine patient management. To this end Cellular Oncology forms a platform of scientific information exchange between molecular biologists and geneticists, technical developers, pathologists, (medical) oncologists and other clinicians involved in the management of cancer patients. In vitro studies are preferentially supported by validations in tumor tissue with clinicopathological associations.
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