{"title":"在各种形式的突触可塑性中,基于激酶和磷酸酶活性的 tau 蛋白磷酸化。","authors":"Burak Tan, Esra Tufan, Özlem Barutçu, Ezgi Aslan-Gülpınar, Nurcan Dursun, Cem Süer","doi":"10.1556/2060.2024.00344","DOIUrl":null,"url":null,"abstract":"<p><p>The aim of this study is to show the relationship between the change in the strengthening of synaptic plasticity and tau phosphorylation and tau-kinases and phosphatase. The averages of the field excitatory-postsynaptic potential (fEPSP) and population spike (PS) in the last 5 min were used as a measure of LTP, LTD and MP. Total and phosphorylated levels of tau, kinases and phosphatases were evaluated by western blot and mRNA levels were evaluated by RT-qPCR. The stimulation of synapses by HFS and LFS+HFS increased the phosphorylation of total-tau and phospho-tau at the Thr181, Ser202/Thr205, Ser396 and Ser416 residues, and these were accompanied by increased enzymatic activity of Akt, ERK1/2. The increased phosphorylation of tau may mediate maintenance of LTP. If the increase in phosphorylation of tau cannot be prevented, together with inhibition of the subsequent LTP, this may indicate that the physiological role of hyperphosphorylated tau in synaptic plasticity may extend to pathological processes.</p>","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":" ","pages":"97-123"},"PeriodicalIF":2.2000,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phosphorylation of tau protein based on the activity of kinases and phosphatases in various forms of synaptic plasticity.\",\"authors\":\"Burak Tan, Esra Tufan, Özlem Barutçu, Ezgi Aslan-Gülpınar, Nurcan Dursun, Cem Süer\",\"doi\":\"10.1556/2060.2024.00344\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The aim of this study is to show the relationship between the change in the strengthening of synaptic plasticity and tau phosphorylation and tau-kinases and phosphatase. The averages of the field excitatory-postsynaptic potential (fEPSP) and population spike (PS) in the last 5 min were used as a measure of LTP, LTD and MP. Total and phosphorylated levels of tau, kinases and phosphatases were evaluated by western blot and mRNA levels were evaluated by RT-qPCR. The stimulation of synapses by HFS and LFS+HFS increased the phosphorylation of total-tau and phospho-tau at the Thr181, Ser202/Thr205, Ser396 and Ser416 residues, and these were accompanied by increased enzymatic activity of Akt, ERK1/2. The increased phosphorylation of tau may mediate maintenance of LTP. If the increase in phosphorylation of tau cannot be prevented, together with inhibition of the subsequent LTP, this may indicate that the physiological role of hyperphosphorylated tau in synaptic plasticity may extend to pathological processes.</p>\",\"PeriodicalId\":20058,\"journal\":{\"name\":\"Physiology international\",\"volume\":\" \",\"pages\":\"97-123\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-03-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physiology international\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1556/2060.2024.00344\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/3/21 0:00:00\",\"PubModel\":\"Print\",\"JCR\":\"Q3\",\"JCRName\":\"PHYSIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiology international","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1556/2060.2024.00344","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/21 0:00:00","PubModel":"Print","JCR":"Q3","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
本研究的目的是显示突触可塑性的加强与 tau 磷酸化、tau 激酶和磷酸酶之间的关系。研究采用了过去5分钟内场兴奋-突触后电位(fEPSP)和群体棘波(PS)的平均值作为LTP、LTD和MP的测量指标。用 Western 印迹法评估 tau、激酶和磷酸酶的总水平和磷酸化水平,用 RT-qPCR 评估 mRNA 水平。HFS和LFS+HFS对突触的刺激增加了总tau和磷酸化tau在Thr181、Ser202/Thr205、Ser396和Ser416残基上的磷酸化,同时Akt和ERK1/2的酶活性也增加了。tau 磷酸化的增加可能会介导 LTP 的维持。如果不能阻止 tau 磷酸化的增加,同时抑制随后的 LTP,这可能表明高磷酸化 tau 在突触可塑性中的生理作用可能延伸到病理过程。
Phosphorylation of tau protein based on the activity of kinases and phosphatases in various forms of synaptic plasticity.
The aim of this study is to show the relationship between the change in the strengthening of synaptic plasticity and tau phosphorylation and tau-kinases and phosphatase. The averages of the field excitatory-postsynaptic potential (fEPSP) and population spike (PS) in the last 5 min were used as a measure of LTP, LTD and MP. Total and phosphorylated levels of tau, kinases and phosphatases were evaluated by western blot and mRNA levels were evaluated by RT-qPCR. The stimulation of synapses by HFS and LFS+HFS increased the phosphorylation of total-tau and phospho-tau at the Thr181, Ser202/Thr205, Ser396 and Ser416 residues, and these were accompanied by increased enzymatic activity of Akt, ERK1/2. The increased phosphorylation of tau may mediate maintenance of LTP. If the increase in phosphorylation of tau cannot be prevented, together with inhibition of the subsequent LTP, this may indicate that the physiological role of hyperphosphorylated tau in synaptic plasticity may extend to pathological processes.
期刊介绍:
The journal provides a forum for important new research papers written by eminent scientists on experimental medical sciences. Papers reporting on both original work and review articles in the fields of basic and clinical physiology, pathophysiology (from the subcellular organization level up to the oranizmic one), as well as related disciplines, including history of physiological sciences, are accepted.