Vera Radisic, Didrik H. Grevskott, Nadja Junghardt, Lise Øvreås, Nachiket P. Marathe
{"title":"耐多药粪肠球菌菌株通过经处理的污水进入挪威海洋环境。","authors":"Vera Radisic, Didrik H. Grevskott, Nadja Junghardt, Lise Øvreås, Nachiket P. Marathe","doi":"10.1002/mbo3.1397","DOIUrl":null,"url":null,"abstract":"<p>This study aimed to understand the antibiotic resistance prevalence among <i>Enterococcus</i> spp. from raw and treated sewage in Bergen city, Norway. In total, 517 <i>Enterococcus</i> spp. isolates were obtained from raw and treated sewage from five sewage treatment plants (STPs) over three sampling occasions, with <i>Enterococcus faecium</i> as the most prevalent (<i>n</i> = 492) species. <i>E. faecium</i> strains (<i>n</i> = 307) obtained from the influent samples, showed the highest resistance against quinupristin/dalfopristin (67.8%). We observed reduced susceptibility to erythromycin (30.6%) and tetracycline (6.2%) in these strains. <i>E. faecium</i> strains (<i>n</i> = 185) obtained from the effluent samples showed highest resistance against quinupristin/dalfopristin (68.1%) and reduced susceptibility to erythromycin (24.9%) and tetracycline (8.6%). We did not detect resistance against last-resort antibiotics, such as linezolid, vancomycin, and tigecycline in any of the strains. Multidrug-resistant (MDR) <i>E. faecium</i> strains were detected in both influent (2.3%) and effluent (2.2%) samples. Whole genome sequencing of the <i>Enterococcus</i> spp. strains (<i>n</i> = 25) showed the presence of several antibiotic resistance genes, conferring resistance against aminoglycosides, tetracyclines, and macrolides, as well as several virulence genes and plasmid replicons. Two sequenced MDR strains from the effluents belonged to the hospital-associated clonal complex 17 and carried multiple virulence genes. Our study demonstrates that clinically relevant MDR <i>Enterococcus</i> spp. strains are entering the marine environment through treated sewage.</p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"13 2","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1397","citationCount":"0","resultStr":"{\"title\":\"Multidrug-resistant Enterococcus faecium strains enter the Norwegian marine environment through treated sewage\",\"authors\":\"Vera Radisic, Didrik H. Grevskott, Nadja Junghardt, Lise Øvreås, Nachiket P. Marathe\",\"doi\":\"10.1002/mbo3.1397\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This study aimed to understand the antibiotic resistance prevalence among <i>Enterococcus</i> spp. from raw and treated sewage in Bergen city, Norway. In total, 517 <i>Enterococcus</i> spp. isolates were obtained from raw and treated sewage from five sewage treatment plants (STPs) over three sampling occasions, with <i>Enterococcus faecium</i> as the most prevalent (<i>n</i> = 492) species. <i>E. faecium</i> strains (<i>n</i> = 307) obtained from the influent samples, showed the highest resistance against quinupristin/dalfopristin (67.8%). We observed reduced susceptibility to erythromycin (30.6%) and tetracycline (6.2%) in these strains. <i>E. faecium</i> strains (<i>n</i> = 185) obtained from the effluent samples showed highest resistance against quinupristin/dalfopristin (68.1%) and reduced susceptibility to erythromycin (24.9%) and tetracycline (8.6%). We did not detect resistance against last-resort antibiotics, such as linezolid, vancomycin, and tigecycline in any of the strains. Multidrug-resistant (MDR) <i>E. faecium</i> strains were detected in both influent (2.3%) and effluent (2.2%) samples. Whole genome sequencing of the <i>Enterococcus</i> spp. strains (<i>n</i> = 25) showed the presence of several antibiotic resistance genes, conferring resistance against aminoglycosides, tetracyclines, and macrolides, as well as several virulence genes and plasmid replicons. Two sequenced MDR strains from the effluents belonged to the hospital-associated clonal complex 17 and carried multiple virulence genes. 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Multidrug-resistant Enterococcus faecium strains enter the Norwegian marine environment through treated sewage
This study aimed to understand the antibiotic resistance prevalence among Enterococcus spp. from raw and treated sewage in Bergen city, Norway. In total, 517 Enterococcus spp. isolates were obtained from raw and treated sewage from five sewage treatment plants (STPs) over three sampling occasions, with Enterococcus faecium as the most prevalent (n = 492) species. E. faecium strains (n = 307) obtained from the influent samples, showed the highest resistance against quinupristin/dalfopristin (67.8%). We observed reduced susceptibility to erythromycin (30.6%) and tetracycline (6.2%) in these strains. E. faecium strains (n = 185) obtained from the effluent samples showed highest resistance against quinupristin/dalfopristin (68.1%) and reduced susceptibility to erythromycin (24.9%) and tetracycline (8.6%). We did not detect resistance against last-resort antibiotics, such as linezolid, vancomycin, and tigecycline in any of the strains. Multidrug-resistant (MDR) E. faecium strains were detected in both influent (2.3%) and effluent (2.2%) samples. Whole genome sequencing of the Enterococcus spp. strains (n = 25) showed the presence of several antibiotic resistance genes, conferring resistance against aminoglycosides, tetracyclines, and macrolides, as well as several virulence genes and plasmid replicons. Two sequenced MDR strains from the effluents belonged to the hospital-associated clonal complex 17 and carried multiple virulence genes. Our study demonstrates that clinically relevant MDR Enterococcus spp. strains are entering the marine environment through treated sewage.
期刊介绍：
MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era.
The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes.
MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to:
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We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses.
The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations.
MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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