{"title":"孟德尔随机研究:肠道微生物群在败血症中的作用--谁是角度?","authors":"Yeping Bian, Jian Xu, Xiaojing Deng, Suming Zhou","doi":"10.33073/pjm-2024-006","DOIUrl":null,"url":null,"abstract":"<p><p>Gut microbiota (GM) is a crucial underlying player during sepsis pathogenesis. However, the causal relationship is unclear and remains to be determined. A two-sample Mendelian randomization study was implemented. The statistical data about sepsis together with GM summarized from genome-wide association studies were evaluated. Instrumental variables were defined as single-nucleotide polymorphisms with prominent correlations with exposure. The inverse-variance-weighted test was employed as a major approach of Mendelian randomization analysis to estimate of causal relationships. The inverse-variance-weighted analysis results demonstrated that at different taxa levels, Actinobacteria and <i>Bifidobacteriaceae</i> influence sepsis. Actinobacteria had negative relationships to sepsis risk at the phylum (β = -0.34, SE = 0.10, <i>p</i> = 0.0008) and class (β = -0.23, SE = 0.07, <i>p</i> = 0.0011) levels in outcome coded ieu-b-69. Actinobacteria at the phylum level (β = -0.22, SE = 0.10, <i>p</i> = 0.027) was also negatively associated with sepsis in outcome coded ieu-b-4980. <i>Bifidobacteriaceae</i> at the order (β = -0.20, SE = 0.06, <i>p</i> = 0.0021), family (β = -0.20, SE = 0.06, <i>p</i> = 0.0021), and genus (β = -0.20, SE = 0.06, <i>p</i> = 0.0007) levels were all negatively correlated with the risk of sepsis in outcome coded ieu-b-69. The results of the Wald ratio model showed that <i>Tyzzerella</i> genus (OR (95%CI) = 0.6902[0.4907,0.9708], <i>p</i> = 0.0331) and Gastranaerophilales order (OR (95%CI) = 0.5907[0.3516,0.9926], <i>p</i> = 0.0468) were negatively connected with sepsis. This study implied at different taxa levels Actinobacteria and <i>Bifidobacteriaceae</i>, <i>Tyzzerella</i> genus, and Gastranaerophilales order have a causal relationship with sepsis, indicating that they are protective factors for the incidence of sepsis.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"73 1","pages":"49-57"},"PeriodicalIF":0.0000,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911657/pdf/","citationCount":"0","resultStr":"{\"title\":\"A Mendelian Randomization Study: Roles of Gut Microbiota in Sepsis - Who is the Angle?\",\"authors\":\"Yeping Bian, Jian Xu, Xiaojing Deng, Suming Zhou\",\"doi\":\"10.33073/pjm-2024-006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Gut microbiota (GM) is a crucial underlying player during sepsis pathogenesis. However, the causal relationship is unclear and remains to be determined. A two-sample Mendelian randomization study was implemented. The statistical data about sepsis together with GM summarized from genome-wide association studies were evaluated. Instrumental variables were defined as single-nucleotide polymorphisms with prominent correlations with exposure. The inverse-variance-weighted test was employed as a major approach of Mendelian randomization analysis to estimate of causal relationships. The inverse-variance-weighted analysis results demonstrated that at different taxa levels, Actinobacteria and <i>Bifidobacteriaceae</i> influence sepsis. Actinobacteria had negative relationships to sepsis risk at the phylum (β = -0.34, SE = 0.10, <i>p</i> = 0.0008) and class (β = -0.23, SE = 0.07, <i>p</i> = 0.0011) levels in outcome coded ieu-b-69. Actinobacteria at the phylum level (β = -0.22, SE = 0.10, <i>p</i> = 0.027) was also negatively associated with sepsis in outcome coded ieu-b-4980. <i>Bifidobacteriaceae</i> at the order (β = -0.20, SE = 0.06, <i>p</i> = 0.0021), family (β = -0.20, SE = 0.06, <i>p</i> = 0.0021), and genus (β = -0.20, SE = 0.06, <i>p</i> = 0.0007) levels were all negatively correlated with the risk of sepsis in outcome coded ieu-b-69. The results of the Wald ratio model showed that <i>Tyzzerella</i> genus (OR (95%CI) = 0.6902[0.4907,0.9708], <i>p</i> = 0.0331) and Gastranaerophilales order (OR (95%CI) = 0.5907[0.3516,0.9926], <i>p</i> = 0.0468) were negatively connected with sepsis. This study implied at different taxa levels Actinobacteria and <i>Bifidobacteriaceae</i>, <i>Tyzzerella</i> genus, and Gastranaerophilales order have a causal relationship with sepsis, indicating that they are protective factors for the incidence of sepsis.</p>\",\"PeriodicalId\":94173,\"journal\":{\"name\":\"Polish journal of microbiology\",\"volume\":\"73 1\",\"pages\":\"49-57\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911657/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polish journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33073/pjm-2024-006\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/3/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polish journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33073/pjm-2024-006","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
A Mendelian Randomization Study: Roles of Gut Microbiota in Sepsis - Who is the Angle?
Gut microbiota (GM) is a crucial underlying player during sepsis pathogenesis. However, the causal relationship is unclear and remains to be determined. A two-sample Mendelian randomization study was implemented. The statistical data about sepsis together with GM summarized from genome-wide association studies were evaluated. Instrumental variables were defined as single-nucleotide polymorphisms with prominent correlations with exposure. The inverse-variance-weighted test was employed as a major approach of Mendelian randomization analysis to estimate of causal relationships. The inverse-variance-weighted analysis results demonstrated that at different taxa levels, Actinobacteria and Bifidobacteriaceae influence sepsis. Actinobacteria had negative relationships to sepsis risk at the phylum (β = -0.34, SE = 0.10, p = 0.0008) and class (β = -0.23, SE = 0.07, p = 0.0011) levels in outcome coded ieu-b-69. Actinobacteria at the phylum level (β = -0.22, SE = 0.10, p = 0.027) was also negatively associated with sepsis in outcome coded ieu-b-4980. Bifidobacteriaceae at the order (β = -0.20, SE = 0.06, p = 0.0021), family (β = -0.20, SE = 0.06, p = 0.0021), and genus (β = -0.20, SE = 0.06, p = 0.0007) levels were all negatively correlated with the risk of sepsis in outcome coded ieu-b-69. The results of the Wald ratio model showed that Tyzzerella genus (OR (95%CI) = 0.6902[0.4907,0.9708], p = 0.0331) and Gastranaerophilales order (OR (95%CI) = 0.5907[0.3516,0.9926], p = 0.0468) were negatively connected with sepsis. This study implied at different taxa levels Actinobacteria and Bifidobacteriaceae, Tyzzerella genus, and Gastranaerophilales order have a causal relationship with sepsis, indicating that they are protective factors for the incidence of sepsis.