由植物性适应原组成的外用精华液的基因表达分析,该精华液旨在支持体内平衡和皮肤质量。

Q2 Medicine Journal of Clinical and Aesthetic Dermatology Pub Date : 2024-02-01
Zoe Diana Draelos, Pearl E Grimes, Jacqueline Watchmaker, Diane B Nelson
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引用次数: 0

摘要

目的:特意开发了一种由植物性适应原组成的外用血清,以支持皮肤适应和实现平衡的能力。本研究评估了局部接触后与皮肤平衡有关的目标基因的表达变化:方法:利用体外表皮模型,在每个组织(N=4)的表面接触 15μL 研究产品(MYS 血清)48 小时后,对基因表达进行定量聚合酶链反应(qPCR)分析。对在皮肤平衡中起关键作用的生物标志物进行了分析:芳基烃受体 (AhR)、氯离子通道附属物 2 (CLCA2)、金属硫蛋白 1A (MT1A)、1F (MT1F) 和 1G (MT1G),以及硫代氧化还原酶 1 (TXNRD1)。采用非配对 t 检验分析法计算具有统计学意义的变化(p2 为具有统计学意义:与对照组相比,暴露于 MYS 血清后 AhR 的表达量增加了 85%(FC=1.85),这表明细胞和表皮的平衡以及皮肤屏障对压力的反应得到了加强。与对照组相比,TXNRD1(293%;FC=3.93)、MT1A(307%;FC=4.07)、MT1F(529%;FC=6.29)和 MT1G(163%;FC=12.63)的表达量出现了统计学意义上的显著增加,表明支持皮肤对压力和免疫平衡的适应性反应。CLCA2水平显著下降(69%;FC=-3.24),表明氧化应激诱导的衰老受到抑制:结论:利用体外表皮皮肤模型,由植物适应原组成的血清显示了目标基因表达的变化,这些基因在皮肤应对压力和实现平衡的能力中发挥着重要作用。
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Gene Expression Analysis of a Topical Serum Comprised of Plant-based Adaptogens Developed to Support Homeostasis and Skin Quality.

Objective: A topical serum comprised of plant-based adaptogens was purposefully developed to support the ability of the skin to adapt and achieve balance. The study described herein evaluated changes in the expression of target genes related to skin homeostasis following topical exposure.

Methods: Utilizing an in vitro epidermal skin model, quantitative polymerase chain reaction (qPCR) analysis of gene expression was conducted following 48-hour exposure to 15μL of the study product (MYS serum) to the surface of each tissue (N=4). Biomarkers that play a key role in skin homeostasis were analyzed: Aryl hydrocarbon receptor (AhR), chloride channel accessory 2 (CLCA2), metallothionein 1A (MT1A), 1F (MT1F), and 1G (MT1G), and thioredoxin reductase 1 (TXNRD1). Statistically significant changes were calculated using unpaired t-test analysis (p<0.05) versus control (saline). A linear Fold Change (FC) value >2 was considered statistically significant.

Results: An 85 percent (FC=1.85) increase in expression of AhR vs. control occurred following exposure to MYS serum indicating enhanced support of cellular and epidermal homeostasis, and the skin barrier's response to stress. Statistically significant increases in expression occurred with TXNRD1 (293%; FC=3.93), MT1A (307%; FC=4.07), MT1F (529%; FC=6.29), and MT1G (163%; FC=12.63) vs. control, indicating support of skin's adaptive response to stress and immune homeostasis. Significantly decreased levels of CLCA2 were demonstrated (69%; FC=-3.24) indicating inhibition of oxidative stress-induced senescence.

Conclusion: Utilizing an in vitro epidermal skin model, a serum comprised of plant-based adaptogens demonstrated changes in the expression of target genes that play important roles in skin's ability to respond to stress and achieve homeostasis.

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104
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