T. Boeselt , P. Terhorst , J. Kroenig , C. Nell , M. Spielmanns , U. Boas , M. Veith , C. Vogelmeier , T. Greulich , AR Koczulla , B. Beutel , J. Huber , H. Heers
{"title":"利用离子迁移谱法对 COVID-19 患者尿液中的挥发性有机化合物进行特定分子峰分析:一种新的诊断方法。","authors":"T. Boeselt , P. Terhorst , J. Kroenig , C. Nell , M. Spielmanns , U. Boas , M. Veith , C. Vogelmeier , T. Greulich , AR Koczulla , B. Beutel , J. Huber , H. Heers","doi":"10.1016/j.jviromet.2024.114910","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p>SARS-CoV-2 is usually diagnosed from naso-/oropharyngeal swabs which are uncomfortable and prone to false results. This study investigated a novel diagnostic approach to Covid-19 measuring volatile organic compounds (VOC) from patients’ urine.</p></div><div><h3>Methods</h3><p>Between June 2020 and February 2021, 84 patients with positive RT-PCR for SARS-CoV-2 were recruited as well as 54 symptomatic individuals with negative RT-PCR. Midstream urine samples were obtained for VOC analysis using ion mobility spectrometry (IMS) which detects individual molecular components of a gas sample based on their size, configuration, and charge after ionization.</p></div><div><h3>Results</h3><p>Peak analysis of the 84 Covid and 54 control samples showed good group separation. In total, 37 individual specific peaks were identified, 5 of which (P134, 198, 135, 75, 136) accounted for significant differences between groups, resulting in sensitivities of 89–94% and specificities of 82–94%. A decision tree was generated from the relevant peaks, leading to a combined sensitivity and specificity of 98% each.</p></div><div><h3>Discussion</h3><p>VOC-based diagnosis can establish a reliable separation between urine samples of Covid-19 patients and negative controls. Molecular peaks which apparently are disease-specific were identified. IMS is an additional non-invasive and cheap device for the diagnosis of this ongoing endemic infection. Further studies are needed to validate sensitivity and specificity.</p></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S016609342400034X/pdfft?md5=7973be6a5159da6ab0dfee1f2f2374a2&pid=1-s2.0-S016609342400034X-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Specific molecular peak analysis by ion mobility spectrometry of volatile organic compounds in urine of COVID-19 patients: A novel diagnostic approach\",\"authors\":\"T. Boeselt , P. Terhorst , J. Kroenig , C. Nell , M. Spielmanns , U. Boas , M. Veith , C. Vogelmeier , T. Greulich , AR Koczulla , B. Beutel , J. Huber , H. 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Specific molecular peak analysis by ion mobility spectrometry of volatile organic compounds in urine of COVID-19 patients: A novel diagnostic approach
Introduction
SARS-CoV-2 is usually diagnosed from naso-/oropharyngeal swabs which are uncomfortable and prone to false results. This study investigated a novel diagnostic approach to Covid-19 measuring volatile organic compounds (VOC) from patients’ urine.
Methods
Between June 2020 and February 2021, 84 patients with positive RT-PCR for SARS-CoV-2 were recruited as well as 54 symptomatic individuals with negative RT-PCR. Midstream urine samples were obtained for VOC analysis using ion mobility spectrometry (IMS) which detects individual molecular components of a gas sample based on their size, configuration, and charge after ionization.
Results
Peak analysis of the 84 Covid and 54 control samples showed good group separation. In total, 37 individual specific peaks were identified, 5 of which (P134, 198, 135, 75, 136) accounted for significant differences between groups, resulting in sensitivities of 89–94% and specificities of 82–94%. A decision tree was generated from the relevant peaks, leading to a combined sensitivity and specificity of 98% each.
Discussion
VOC-based diagnosis can establish a reliable separation between urine samples of Covid-19 patients and negative controls. Molecular peaks which apparently are disease-specific were identified. IMS is an additional non-invasive and cheap device for the diagnosis of this ongoing endemic infection. Further studies are needed to validate sensitivity and specificity.
期刊介绍:
The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery.
The methods may include, but not limited to, the study of:
Viral components and morphology-
Virus isolation, propagation and development of viral vectors-
Viral pathogenesis, oncogenesis, vaccines and antivirals-
Virus replication, host-pathogen interactions and responses-
Virus transmission, prevention, control and treatment-
Viral metagenomics and virome-
Virus ecology, adaption and evolution-
Applied virology such as nanotechnology-
Viral diagnosis with novelty and comprehensive evaluation.
We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.