评估苁蓉对 Wistar 大鼠缺血再灌注损伤的保肝作用

IF 3.7 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Annals of hepatology Pub Date : 2024-02-01 DOI:10.1016/j.aohep.2024.101417
Evelyn L. García-Carmona , Ramiro Tijerina-Márquez , Liliana Torres-González , Diana Moreno-Peña , Diana R. Rodríguez-Rodríguez , Paulina Espíndola-Vela , Linda E. Muñoz-Espinosa , Edelmiro Pérez-Rodríguez , Homero Zapata-Chavira , Paula Cordero-Pérez
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引用次数: 0

摘要

导言和目的肝移植是治疗不可逆性肝损伤患者的最佳方法。移植的主要并发症是缺血再灌注损伤(I/R),它会诱发原发性移植排斥反应。由于植物提取物具有潜在的抗炎和抗氧化活性,因此在 I/R 前使用植物提取物治疗可降低这种损伤的严重程度。一种具有潜在抗氧化活性的植物是Flourensia cernua(Fc)。研究目的是评估 Fc 对 Wistar 大鼠缺血再灌注引起的肝损伤的保护作用。给大鼠注射 Fc(200 mg/kg/,p.o/5 天),然后夹闭左侧门静脉三联体,造成 1 小时 70% 的缺血和 2 或 24 小时的再灌注。测定生化和氧化应激生物标志物、促炎细胞因子和基因表达。伦理委员会批准了 HI17-00002 登记和 PAICYT 152-CS-2022 资助。研究小组声明无利益冲突。结果与 Sham(SH)相比,再灌注 2 小时(IR2hr)和 24 小时(IR24hr)的 I/R 组显示 ALT 和 AST 浓度显著升高;只有 FcIR2hr 能显著降低这些酶(图 1)。其余的生化指标在各组之间没有任何明显差异。IR2hr 组诱导氧化应激生物标志物发生了统计学意义上的显著变化,而 Fc 则抵消了这些影响,降低了丙二醛(MDA),增加了还原型谷胱甘肽(GSH)和超氧化物歧化酶(SOD)(图 2)。在 IR2hr 组中,NFκβ 的基因表达增加,而 F. cernua 可以抵消这种增加。结论I/R是一种被广泛研究的损伤模型,能够诱导多个领域的病理变化,与本研究中观察到的结果并不相同;Fc的水醇提取物在200毫克/千克时显示出抗炎和抗氧化活性,它没有毒性,并被证明对I/R具有肝保护作用。
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Evaluation of the hepatoprotective effect of Flourensia cernua against the damage induced ischemia-reperfusion in Wistar rats.

Introduction and Objectives

Liver transplantation is the optimal treatment in patients with irreversible liver damage. The principal complication of a transplant is ischemia-reperfusion injury (I/R), which induces primary graft rejection. Treatment with plant extracts prior to I/R has decreased the severity of this injury due to their potential anti-inflammatory and antioxidant activity. A plant that presents potential antioxidant activity is Flourensia cernua (Fc). The objective was to evaluate the hepatoprotective effect of Flourensia cernua against the damage induced by ischemia-reperfusion in Wistar rats.

Materials an Patients

42 mixed Wistar rats were sorted into 7 groups (n=6). Fc was administered (200 mg/kg/, p.o/5 days) followed by I/R clamping of the left portal triad producing 1hr of 70% ischemia and 2 or 24hrs of reperfusion. Biochemical and oxidative stress biomarkers, proinflammatory cytokine and gene expression were determined. Ethics Committee approval under HI17-00002 registry and PAICYT 152-CS-2022 financing. The research group declares no conflict of interest.

Results

The I/R groups with 2 (IR2hr) and 24 hour (IR24hr) reperfusion displayed significantly elevated ALT and AST concentrations vs. Sham (SH); only FcIR2hr significantly decreased these enzymes (Figure 1). The remaining biochemical parameters did not show any significant differences between the groups. IR2hr group induced a statistically significant alteration of oxidative stress biomarkers, Fc counteracted these effects, with a decrease of malondialdehyde(MDA) and an increase of reduced glutathione (GSH) and the superoxide dismutase(SOD) (Figure 2). The gene expression of NFκβ was increased in IR2hr group, the treatment with F. cernua counteracted this increase. TNF-α was significantly increased in the IR2hr group and decreased in the treatment group.

Conclusions

I/R is a widely studied injury model, capable of inducing pathological changes in several spheres, not unlike the observed results in the present study; the hydroalcoholic extract of Fc displayed anti-inflammatory and antioxidant activity at 200mg/kg, it was not toxic and proved to be hepatoprotective against I/R.

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来源期刊
Annals of hepatology
Annals of hepatology 医学-胃肠肝病学
CiteScore
7.90
自引率
2.60%
发文量
183
审稿时长
4-8 weeks
期刊介绍: Annals of Hepatology publishes original research on the biology and diseases of the liver in both humans and experimental models. Contributions may be submitted as regular articles. The journal also publishes concise reviews of both basic and clinical topics.
期刊最新文献
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