褪黑素和二乙基二硫代氨基甲酸盐对小鼠 N1E-115 神经母细胞瘤细胞（克隆 C-1300）的联合作用

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2024-03-07 DOI:10.1134/s0006350923050184

S. N. Myakisheva, Y. L. Baburina, M. I. Kobyakova, R. R. Krestinin, L. D. Sotnikova, O. V. Krestinina

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引用次数: 0

摘要

摘要研究了二乙基二硫代氨基甲酸钠、褪黑素及其联合使用对小鼠 N1E-115 神经母细胞瘤细胞（克隆 C-1300）培养过程中的增殖活性、细胞膜 Ca2+、膜电位变化和活性氧产生的影响。二乙基二硫代氨基甲酸钠和褪黑素具有抑制细胞增殖和促进细胞分化的作用。与此同时，抗凋亡蛋白 Bcl-2 的含量下降，而促凋亡蛋白 Bax 的含量上升，这可能表明细胞凋亡级联已经启动。然而，同时使用这两种药物并不能增强观察到的效果。显然，二乙基二硫代氨基甲酸盐和褪黑素对细胞的作用机制是不同的。

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The Combined Effects of Melatonin and Diethyldithiocarbamate on Mouse N1E-115 Neuroblastoma Cells (Clone C-1300)

Abstract

The effects of sodium diethyldithiocarbamate, melatonin, and their combined use on proliferative activity, changes in cytosolic Ca²⁺, membrane potential, and production of reactive oxygen species in the culture of mouse N1E-115 neuroblastoma cells (clone C-1300) were studied. Sodium diethyldithiocarbamate and melatonin have been shown to inhibit proliferation and enhance cell differentiation. At the same time, the content of the anti-apoptotic protein Bcl-2 decreased, while the content of the pro-apoptotic protein Bax increased, which might suggest the launch of an apoptotic cascade. However, the use of these two drugs together did not enhance the observed effects. Apparently, the mechanisms by which diethyldithiocarbamates and melatonin acted on cells were different.

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.