{"title":"茄科植物多糖的成分和有丝分裂活性","authors":"E. A. Generalov, L. V. Yakovenko","doi":"10.1134/S0006350923050093","DOIUrl":null,"url":null,"abstract":"<p>The STP polysaccharide was isolated from an aqueous extract of <i>Solanum tuberosum</i> L. and purified by ion exchange chromatography and gel filtration. Its molecular mass was determined by gel-penetrating chromatography and high-performance liquid chromatography, and its monosaccharide composition was analyzed by high-performance liquid chromatography and gas chromatography using a flame ionization detector and a capillary column. It was shown that STP polysaccharide consists of galactose and arabinose at 37.5% and 23.5%, respectively, as well as uronic acids (9.7%), glucose monosaccharide residues (15%), and proteins (at least 9%). The molecular mass of STP is 70 kDa. The method of IR-Fourier spectroscopy was used for the structural analysis of STP. The mitogenic activity of the extracted polysaccharide was comparable to the activity of lipopolysaccharide.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":null,"pages":null},"PeriodicalIF":4.0330,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Composition and Mitogenic Activity of Polysaccharide from Solanum tuberosum L.\",\"authors\":\"E. A. Generalov, L. V. Yakovenko\",\"doi\":\"10.1134/S0006350923050093\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The STP polysaccharide was isolated from an aqueous extract of <i>Solanum tuberosum</i> L. and purified by ion exchange chromatography and gel filtration. Its molecular mass was determined by gel-penetrating chromatography and high-performance liquid chromatography, and its monosaccharide composition was analyzed by high-performance liquid chromatography and gas chromatography using a flame ionization detector and a capillary column. It was shown that STP polysaccharide consists of galactose and arabinose at 37.5% and 23.5%, respectively, as well as uronic acids (9.7%), glucose monosaccharide residues (15%), and proteins (at least 9%). The molecular mass of STP is 70 kDa. The method of IR-Fourier spectroscopy was used for the structural analysis of STP. The mitogenic activity of the extracted polysaccharide was comparable to the activity of lipopolysaccharide.</p>\",\"PeriodicalId\":493,\"journal\":{\"name\":\"Biophysics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.0330,\"publicationDate\":\"2024-03-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biophysics\",\"FirstCategoryId\":\"4\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S0006350923050093\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysics","FirstCategoryId":"4","ListUrlMain":"https://link.springer.com/article/10.1134/S0006350923050093","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
The Composition and Mitogenic Activity of Polysaccharide from Solanum tuberosum L.
The STP polysaccharide was isolated from an aqueous extract of Solanum tuberosum L. and purified by ion exchange chromatography and gel filtration. Its molecular mass was determined by gel-penetrating chromatography and high-performance liquid chromatography, and its monosaccharide composition was analyzed by high-performance liquid chromatography and gas chromatography using a flame ionization detector and a capillary column. It was shown that STP polysaccharide consists of galactose and arabinose at 37.5% and 23.5%, respectively, as well as uronic acids (9.7%), glucose monosaccharide residues (15%), and proteins (at least 9%). The molecular mass of STP is 70 kDa. The method of IR-Fourier spectroscopy was used for the structural analysis of STP. The mitogenic activity of the extracted polysaccharide was comparable to the activity of lipopolysaccharide.
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍:
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.