Jung-Hyang Park, Eunbin Kwag, Mi-Kyung Jeong, So-Jung Park, Sanghun Lee, Hwa-Seung Yoo
{"title":"全基因组分析发现 SEMA4D 是非小细胞肺癌患者温度敏感性的新型候选基因","authors":"Jung-Hyang Park, Eunbin Kwag, Mi-Kyung Jeong, So-Jung Park, Sanghun Lee, Hwa-Seung Yoo","doi":"10.1177/15347354241233544","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>In the era of precision medicine, individual temperature sensitivity has been highlighted. This trait has traditionally been used for cold-heat pattern identification to understand the inherent physical characteristics, which are influenced by genetic factors, of an individual. However, genome-wide association studies (GWASs) on this trait are limited.</p><p><strong>Methods: </strong>Using genotype data from 90 patients with advanced non-small cell lung cancer (NSCLC) and epidermal growth factor receptor mutations, we performed a GWAS to assess the association between single nucleotide polymorphisms (SNPs) and temperature sensitivity, such as cold and heat scores. The score of each participant was evaluated using self-administered questionnaires on common symptoms and a 15-item symptom-based cold-heat pattern identification questionnaire.</p><p><strong>Results: </strong>The GWAS was adjusted for confounding factors, including age and sex, and significant associations were identified for cold and heat scores: SNP rs145814326, located on the intron of <i>SORCS2</i> at chromosome 4p16.1, had a <i>P</i>-value of 1.86 × 10<sup>-7</sup>; and SNP rs79297667, located upstream from <i>SEMA4D</i> at chromosome 9q22.2, had a <i>P</i>-value of 8.97 × 10<sup>-8</sup>. We also found that the genetic variant regulates the expression level of <i>SEMA4D</i> in the main tissues, including the lungs and white blood cells, in NSCLC.</p><p><strong>Conclusions: </strong><i>SEMA4D</i> was found to be significantly associated with temperature sensitivity in patients with NSCLC, suggesting an increased expression of <i>SEMA4D</i> in patients with higher heat scores. The potential role of temperature sensitivity as a prognostic or predictive marker of immune response in NSCLC should be further studied.</p>","PeriodicalId":13734,"journal":{"name":"Integrative Cancer Therapies","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10935759/pdf/","citationCount":"0","resultStr":"{\"title\":\"Genome-wide Analysis Identified <i>SEMA4D</i>, Novel Candidate Gene for Temperature Sensitivity in Patients With Non-Small Cell Lung Cancer.\",\"authors\":\"Jung-Hyang Park, Eunbin Kwag, Mi-Kyung Jeong, So-Jung Park, Sanghun Lee, Hwa-Seung Yoo\",\"doi\":\"10.1177/15347354241233544\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>In the era of precision medicine, individual temperature sensitivity has been highlighted. This trait has traditionally been used for cold-heat pattern identification to understand the inherent physical characteristics, which are influenced by genetic factors, of an individual. However, genome-wide association studies (GWASs) on this trait are limited.</p><p><strong>Methods: </strong>Using genotype data from 90 patients with advanced non-small cell lung cancer (NSCLC) and epidermal growth factor receptor mutations, we performed a GWAS to assess the association between single nucleotide polymorphisms (SNPs) and temperature sensitivity, such as cold and heat scores. The score of each participant was evaluated using self-administered questionnaires on common symptoms and a 15-item symptom-based cold-heat pattern identification questionnaire.</p><p><strong>Results: </strong>The GWAS was adjusted for confounding factors, including age and sex, and significant associations were identified for cold and heat scores: SNP rs145814326, located on the intron of <i>SORCS2</i> at chromosome 4p16.1, had a <i>P</i>-value of 1.86 × 10<sup>-7</sup>; and SNP rs79297667, located upstream from <i>SEMA4D</i> at chromosome 9q22.2, had a <i>P</i>-value of 8.97 × 10<sup>-8</sup>. We also found that the genetic variant regulates the expression level of <i>SEMA4D</i> in the main tissues, including the lungs and white blood cells, in NSCLC.</p><p><strong>Conclusions: </strong><i>SEMA4D</i> was found to be significantly associated with temperature sensitivity in patients with NSCLC, suggesting an increased expression of <i>SEMA4D</i> in patients with higher heat scores. The potential role of temperature sensitivity as a prognostic or predictive marker of immune response in NSCLC should be further studied.</p>\",\"PeriodicalId\":13734,\"journal\":{\"name\":\"Integrative Cancer Therapies\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10935759/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Integrative Cancer Therapies\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/15347354241233544\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Integrative Cancer Therapies","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/15347354241233544","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
引用次数: 0
摘要
背景:在精准医疗时代,个体对温度的敏感性受到重视。这一特征历来被用于冷热模式识别,以了解个体受遗传因素影响的固有生理特征。然而,有关这一特征的全基因组关联研究(GWAS)却十分有限:我们利用 90 名晚期非小细胞肺癌(NSCLC)患者的基因型数据和表皮生长因子受体突变,开展了一项 GWAS 研究,以评估单核苷酸多态性(SNPs)与温度敏感性(如冷热评分)之间的关联。我们使用常见症状自填问卷和基于症状的 15 项冷热模式识别问卷对每位参与者的得分进行了评估:GWAS对年龄和性别等混杂因素进行了调整,结果发现冷热评分存在显著关联:位于染色体 4p16.1 的 SORCS2 内含子上的 SNP rs145814326 的 P 值为 1.86 × 10-7;位于染色体 9q22.2 的 SEMA4D 上游的 SNP rs79297667 的 P 值为 8.97 × 10-8。我们还发现,该遗传变异调控了 SEMA4D 在 NSCLC 主要组织(包括肺和白细胞)中的表达水平:结论:研究发现,SEMA4D与NSCLC患者的温度敏感性显著相关,表明SEMA4D在热评分较高的患者中表达增加。应进一步研究温度敏感性作为NSCLC免疫反应的预后或预测标志物的潜在作用。
Genome-wide Analysis Identified SEMA4D, Novel Candidate Gene for Temperature Sensitivity in Patients With Non-Small Cell Lung Cancer.
Background: In the era of precision medicine, individual temperature sensitivity has been highlighted. This trait has traditionally been used for cold-heat pattern identification to understand the inherent physical characteristics, which are influenced by genetic factors, of an individual. However, genome-wide association studies (GWASs) on this trait are limited.
Methods: Using genotype data from 90 patients with advanced non-small cell lung cancer (NSCLC) and epidermal growth factor receptor mutations, we performed a GWAS to assess the association between single nucleotide polymorphisms (SNPs) and temperature sensitivity, such as cold and heat scores. The score of each participant was evaluated using self-administered questionnaires on common symptoms and a 15-item symptom-based cold-heat pattern identification questionnaire.
Results: The GWAS was adjusted for confounding factors, including age and sex, and significant associations were identified for cold and heat scores: SNP rs145814326, located on the intron of SORCS2 at chromosome 4p16.1, had a P-value of 1.86 × 10-7; and SNP rs79297667, located upstream from SEMA4D at chromosome 9q22.2, had a P-value of 8.97 × 10-8. We also found that the genetic variant regulates the expression level of SEMA4D in the main tissues, including the lungs and white blood cells, in NSCLC.
Conclusions: SEMA4D was found to be significantly associated with temperature sensitivity in patients with NSCLC, suggesting an increased expression of SEMA4D in patients with higher heat scores. The potential role of temperature sensitivity as a prognostic or predictive marker of immune response in NSCLC should be further studied.
期刊介绍:
ICT is the first journal to spearhead and focus on a new and growing movement in cancer treatment. The journal emphasizes scientific understanding of alternative medicine and traditional medicine therapies, and their responsible integration with conventional health care. Integrative care includes therapeutic interventions in diet, lifestyle, exercise, stress care, and nutritional supplements, as well as experimental vaccines, chrono-chemotherapy, and other advanced treatments. Contributors are leading oncologists, researchers, nurses, and health-care professionals.