猫外周血中细胞外囊泡介导的微RNA与Ω干扰素的抗病毒作用之间的相关性

IF 1.9 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Interferon and Cytokine Research Pub Date : 2024-03-01 DOI:10.1089/jir.2023.0174
Mingxing Yang, Guowei Xu, Jingyan Zhang, Zhiting Guo, Chao Liang, Yajun Li, Lei Wang, Yuxia Zhou, Yi Ru, Jianxi Li, Xuezhi Wang, Yan Sun
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引用次数: 0

摘要

猫干扰素ω(IFN-ω)已被证实具有很高的抗病毒活性,但其深层次的抗病毒作用仍不为人知。细胞外囊泡(EVs)已被证实可通过各种活性物质,特别是通过其携带的微RNA(miRNA)参与机体免疫应答途径的调节。本研究采用差速离心法从猫外周血中分离出EVs,进一步发现IFN-ω处理后24小时内EVs中IFN-ω的含量持续增加,IFN-ω处理后12小时内EVs中大量miRNA显著下调。这些明显差异表达的 miRNA 对调节抗病毒细胞因子的变化非常重要。本研究首次揭示了猫外周血中EVs介导的miRNA与IFN-ω对抗病毒免疫应答的相关性,为开发新型抗病毒纳米药物和研究IFN-ω的抗病毒作用提供了有力的数据支持。
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Correlation Between MicroRNA by Extracellular Vesicle Mediated and Antiviral Effects of Interferon Omega in Feline Peripheral Blood.

Feline interferon omega (IFN-ω) has been proven to have high antiviral activity; however, its in-depth antiviral effects remain unknown. Extracellular vesicles (EVs) have been demonstrated to participate in the regulation of the immune response pathway for the body through various active substances, especially through the microRNA (miRNA) carried by them. In this study, we isolated EVs from feline peripheral blood by differential centrifugation, and further found that the content of IFN-ω in EVs increased continuously within 24 h after IFN-ω treatment, and a large number of miRNAs were significantly downregulated in EVs within 12 h after IFN-ω treatment. These significantly differentially expressed miRNAs were important for regulating changes in antiviral cytokines. This study reveals for the first time the correlation between EVs-mediated miRNA in feline peripheral blood and IFN-ω on antiviral immune response, which may provide strong data support for the development of novel antiviral nanomedicine and the research of the antiviral effects of IFN-ω.

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来源期刊
CiteScore
3.80
自引率
0.00%
发文量
78
审稿时长
2.2 months
期刊介绍: Journal of Interferon & Cytokine Research (JICR) provides the latest groundbreaking research on all aspects of IFNs and cytokines. The Journal delivers current findings on emerging topics in this niche community, including the role of IFNs in the therapy of diseases such as multiple sclerosis, the understanding of the third class of IFNs, and the identification and function of IFN-inducible genes.
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