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引用次数: 0
摘要
背景及时的疟疾治疗和监测是准确诊断疟原虫疟疾的关键。在大多数疟疾流行地区,金标准显微镜检查已被广泛用于疟疾诊断。但在镜下和无症状的情况下,显微镜诊断就会受到质疑。本研究旨在开发一种简单、经济、可靠的核酸扩增技术,用于检测疟原虫。在苏拉特拉克塔丹研究中心(Surat Raktadan Kendra & Research Centre- Blood Bank)的现有设施中,通过制备厚膜对每个样本进行显微镜初筛。常规 PCR(聚合酶链式反应)用于针对疟原虫的 18 S rRNA 基因进行种属特异性扩增。研究结果表明,巢式 PCR 不仅能检测出所有显微镜下的阳性样本,还能检测出显微镜下漏检或误检的亚显微感染。因此,与显微镜检查相比,分子检测技术的灵敏度更高。
Confirmatory Assay for Laboratory Diagnosis of Malaria Using Molecular Approach
Background
Prompt malarial treatment and surveillance is crucial for accurate diagnosis of Plasmodium Sp. Gold standard microscopic examination has been widely applied for diagnosis of malaria in most part of the endemic areas. But in case of submicroscopic and asymptomatic microscopic diagnosis is questioned. The study aims to develop a simple, cost effective & robust nucleic acid amplification technique for the detection of malaria parasite.
Methods
Study population included 50 clinically diagnosed positive malaria patient samples from various pathological laboratories. Microscopy by preparing thick film was carried out of every sample for primary screening in the available facility of Surat Raktadan Kendra & Research Centre- Blood Bank. The conventional PCR (Polymerase Chain Reaction) was applied for genus-specific amplification targeting the 18 S rRNA gene of Plasmodium. Agarose gel electrophoresis was used to separate and analyze the amplified PCR product using 2% Agarose gel.
Results and Conclusion
The study shows that nested PCR not only detected all microscopic positive samples, but also detected submicroscopic infections that were missed or misread by microscopy. Hence, the sensitivity of molecular based detection technique is proved to be more compared to microscopic examination.
期刊介绍:
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.